Stevenson B, Tilly K, Rosa P A
Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA.
J Bacteriol. 1996 Jun;178(12):3508-16. doi: 10.1128/jb.178.12.3508-3516.1996.
We have identified four loci in Borrelia burgdorferi B31 that contain open reading frames capable of encoding six proteins that are related to the antigenic proteins OspE and OspF. We have designated these proteins Erp, for OspEF-related protein, and named their respective genes erp. The erpA and erpB genes are linked, as are erpC and erpD, and the pairs probably constitute two operons. The erpG and erpH genes appear to be monocistronic. The ErpA and ErpC proteins are expressed by B. burgdorferi B31 in culture and are recognized by a polyclonal antiserum raised against the OspE protein of B. burgdorferi N40. The four erp loci are each located on different 32-kb circular plasmids that contain additional DNA sequences that are homologous to each other and to an 8.3-kb circular plasmid of B. burgdorferi sensu lato Ip2l. All four 32-kb plasmids can be maintained within a single bacterium, which may provide a model for the study of plasmid replication and segregation in B. burgdorferi.
我们在伯氏疏螺旋体B31中鉴定出了四个基因座，这些基因座包含能够编码六种与抗原蛋白OspE和OspF相关的蛋白质的开放阅读框。我们将这些蛋白质命名为Erp，即OspEF相关蛋白，并将它们各自的基因命名为erp。erpA和erpB基因是连锁的，erpC和erpD也是连锁的，这两对基因可能构成两个操纵子。erpG和erpH基因似乎是单顺反子的。ErpA和ErpC蛋白在培养的伯氏疏螺旋体B31中表达，并被针对伯氏疏螺旋体N40的OspE蛋白产生的多克隆抗血清所识别。这四个erp基因座分别位于不同的32 kb环形质粒上，这些质粒包含彼此同源且与伯氏疏螺旋体狭义种Ip2l的一个8.3 kb环形质粒同源的其他DNA序列。所有四个32 kb质粒都可以在单个细菌中维持，这可能为研究伯氏疏螺旋体中的质粒复制和分离提供一个模型。
