Woźny W, Piasek A, Elbaum D
Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland.
Viral Immunol. 1997;10(2):73-82. doi: 10.1089/vim.1997.10.73.
Several tests are currently available to assist in the diagnosis of the hepatitis C virus (HCV) and human immunodeficiency virus (HIV). Tests that actually detect or quantify these viruses are based on the polymerase chain reaction (PCR) technique. However, the application of PCR is limited by the cost, labor, time-consumption, and potential for contamination. In this article we describe some procedures developed to reduce these limitations. We have developed and validated simultaneous detection methods for HIV RNA and HCV RNA in single serum samples using Amplicor PCR tests. The sensitivity and specificity of this method are comparable with the results obtained with commercial reverse transcription polymerase chain reaction (RT-PCR) techniques for HIV and HCV RNA detection. In addition we have modified the HIV Amplicor test for the RT-PCR procedure and the Chomczynski's method of RNA isolation. We hope that our method can find same applications in HIV and HCV coinfection research, blood screening, and medical diagnosis.
目前有几种检测方法可用于辅助诊断丙型肝炎病毒（HCV）和人类免疫缺陷病毒（HIV）。实际检测或定量这些病毒的检测方法基于聚合酶链反应（PCR）技术。然而，PCR的应用受到成本、人力、时间消耗以及污染可能性的限制。在本文中，我们描述了一些为减少这些限制而开发的程序。我们已经开发并验证了使用Amplicor PCR检测法同时检测单份血清样本中HIV RNA和HCV RNA的方法。该方法的灵敏度和特异性与用于HIV和HCV RNA检测的商业逆转录聚合酶链反应（RT-PCR）技术所获得的结果相当。此外，我们对HIV Amplicor检测法的RT-PCR程序和Chomczynski的RNA分离方法进行了改进。我们希望我们的方法能够在HIV和HCV合并感染研究、血液筛查及医学诊断中找到相同的应用。
