Chen J X, Kagan J
Department of Chemistry, University of Illinois at Chicago 60607-7003, USA.
J Photochem Photobiol B. 1997 May;39(1):56-62. doi: 10.1016/s1011-1344(97)83322-2.
7-Methylpyrido[3,4-c]psoralen has recently been found to photosensitize the crosslinking and cleavage of double-stranded pBR322 DNA (4361 bp) with an unusual degree of site specificity. Following cleavage of the photosensitized pBR322 with the restriction enzymes Pvu II and BamH I, the main fragments were analyzed electrophoretically. Only three possible cleavage areas on the plasmid were suggested by this analysis. They were within the ranges bounded by the positions 801-926, 3172-3303 and 4226-4310. The sensitizer also induced selective cleavage in the related plasmid pUC19 (2686 bp), but the pattern was somewhat more complicated and was not analyzed in detail.
最近发现，7-甲基吡啶并[3,4-c]补骨脂素能够使双链pBR322 DNA（4361碱基对）发生交联和切割，且具有不同寻常程度的位点特异性。在用限制性内切酶Pvu II和BamH I切割经光敏化处理的pBR322之后，对主要片段进行了电泳分析。该分析仅表明质粒上有三个可能的切割区域。它们位于801-926、3172-3303和4226-4310位置所界定的范围内。该敏化剂还能在相关质粒pUC19（2686碱基对）中诱导选择性切割，但切割模式更为复杂，未进行详细分析。
