Loos W J, Verweij J, Nooter K, Stoter G, Sparreboom A
Department of Medical Oncology, Rotterdam Cancer Institute (Daniel den Hoed Kliniek) and University Hospital Rotterdam, Netherlands.
J Chromatogr B Biomed Sci Appl. 1997 Jun 6;693(2):437-41. doi: 10.1016/s0378-4347(97)00089-3.
A sensitive reversed-phase high-performance liquid chromatographic method has been developed and validated for the quantitative determination of docetaxel (I) in human plasma. The concentrations in plasma, for validation procedures spiked with known amounts of I, are read from calibration curves in the range of 10-20,000 ng/ml. The sample preparation involved a liquid-liquid extraction of 1000 microl of sample with a mixture of acetonitrile-n-butylchloride (1:4, v/v). The related compound paclitaxel (II) was used as internal standard. Chromatographic separations were performed an Inertsil ODS-80A column, with UV detection performed at 230 nm. The overall extraction recoveries were 84.3 and 90.0% for I and II, respectively. The lower limit of quantitation was 10 ng/ml, and the accuracy, within-run and between-run precisions at three tested concentrations fell within the generally accepted criteria for bioanalytical assays.
已开发并验证了一种灵敏的反相高效液相色谱法，用于定量测定人血浆中的多西他赛（I）。对于加有已知量I的验证程序，血浆中的浓度可从10 - 20,000 ng/ml范围内的校准曲线读取。样品制备包括用乙腈 - 正丁基氯（1:4，v/v）混合物对1000微升样品进行液 - 液萃取。相关化合物紫杉醇（II）用作内标。色谱分离在Inertsil ODS - 80A柱上进行，紫外检测在230 nm处进行。I和II的总萃取回收率分别为84.3%和90.0%。定量下限为10 ng/ml，在三个测试浓度下的准确度、批内和批间精密度均符合生物分析测定的普遍接受标准。
