Sparreboom Alex, Zhao Ming, Brahmer Julie R, Verweij Jaap, Baker Sharyn D
Laboratory of Experimental Chemotherapy and Pharmacology, Department of Medical Oncology, Rotterdam Cancer Institute (Daniel den Hoed Kliniek) and University Hospital Rotterdam, Groene Hilledijk 301, The Netherlands.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Jun 25;773(2):183-90. doi: 10.1016/s1570-0232(02)00167-8.
A new simple method was developed for the quantitative determination of the docetaxel (Taxotere) vehicle, polysorbate 80 (Tween 80), in human plasma. Calibration curves were constructed in the range of 1-100 microg/ml, using paclitaxel (0.01 mM) as internal standard, and were analyzed using a power fit with equal weighting. Sample pretreatment involved a one-step extraction with acetonitrile-n-butyl chloride (1:4, v/v). The analytes were separated on a Waters X-Terra MS column (50x2.1 mm I.D.) packed with 3.5-microm ODS material, and eluted with methanol-water (9:1, v/v) containing 0.1% formic acid. The column effluent was monitored by tandem mass spectrometry with electrospray ionization. The overall extraction efficiency was 50-60%, with values for precision and accuracy of < or =16% and <15% relative error, respectively. Our current method is approximately 60-100-fold more sensitive than previous assays, and will be used to define Tween 80 disposition in patients receiving Taxotere.
开发了一种新的简单方法用于定量测定人血浆中多西他赛（泰索帝）的溶媒聚山梨酯80（吐温80）。以紫杉醇（0.01 mM）为内标，在1 - 100 μg/ml范围内构建校准曲线，并采用等权重幂拟合进行分析。样品预处理包括用乙腈 - 正丁基氯（1:4，v/v）一步萃取。分析物在填充有3.5-μm ODS材料的沃特世X-Terra MS柱（内径50x2.1 mm）上分离，并用含0.1%甲酸的甲醇 - 水（9:1，v/v）洗脱。柱流出物通过电喷雾电离串联质谱进行监测。总体萃取效率为50 - 60%，精密度值≤16%，相对误差<15%。我们目前的方法比以前的测定方法灵敏度高约60 - 100倍，将用于确定接受泰索帝治疗患者体内吐温80的处置情况。
