Koistinen P, Säily M, Poromaa N, Savolainen E R
Department of Internal Medicine, University of Oulu, Finland.
Acta Haematol. 1997;98(1):14-21. doi: 10.1159/000203547.
The present in vitro study shows how interleukin (IL)-6 modulates clonogenic blast cell growth in complex ways in acute myeloblastic leukemia when used either as a single factor or in different hematopoietic growth factor combinations. In the presence of IL-6, the colony numbers in culture assay decreased to 50 +/- 29% from the basal values (p < 0.001) in 10 cases and increased to 384 +/- 278% of the basal values (p < 0.01) in 5. The inhibitory effect of IL-6 on blast cell colony formation was retained when IL-6 was combined with granulocyte colony stimulating factor, but was lost if IL-6 was used in combination with mast cell growth factor, IL-3, granulocyte-macrophage colony stimulating factor, or IL-4. The stimulatory effect of IL-6 was diminished in the presence of granulocyte colony stimulating factor, but preserved in the presence of other growth factor combinations. IL-6 had a neutral effect on colony growth in 7 cases with acute myeloblastic leukemia. In these cases, however, IL-6 stimulated significantly clonogenic cell growth if combined with mast cell growth factor or granulocyte-macrophage colony stimulating factor.
目前的体外研究表明，在急性髓性白血病中，白细胞介素（IL）-6作为单一因子或与不同造血生长因子联合使用时，以复杂的方式调节原始细胞集落生长。在存在IL-6的情况下，10例患者培养试验中的集落数从基础值降至50±29%（p<0.001），5例患者则增至基础值的384±278%（p<0.01）。当IL-6与粒细胞集落刺激因子联合使用时，其对原始细胞集落形成的抑制作用得以保留，但如果IL-6与肥大细胞生长因子、IL-3、粒细胞-巨噬细胞集落刺激因子或IL-4联合使用，则该抑制作用消失。在存在粒细胞集落刺激因子的情况下，IL-6的刺激作用减弱，但在存在其他生长因子组合时得以保留。IL-6对7例急性髓性白血病患者的集落生长具有中性作用。然而，在这些病例中，如果IL-6与肥大细胞生长因子或粒细胞-巨噬细胞集落刺激因子联合使用，则可显著刺激克隆细胞生长。
