Bogitsh B J, Ribeiro-Rodrigues R, Carter C E
Department of Biology, Vanderbilt University, Nashville, TN 37235, USA.
Parasitol Res. 1997;83(6):624-6. doi: 10.1007/s004360050308.
Incubation for 24 h in culture medium containing 50 mM adenosine triphosphate (ATP) produces distinct alterations in the ultrastructure of Trypanosoma cruzi epimastigotes, most obvious of which is the formation of large membrane-bound vacuoles in the cytosol. These vacuoles become positive following exposure to the macromolecule horseradish peroxidase (HRP). After a 20-min chase in phosphate-buffered saline (PBS) the HRP-positive vacuoles begin to separate into discrete structures such that after a 60-min chase, obvious reservosomes are identifiable. It is hypothesized that extracellular ATP causes increased permeability of the epimastigote's plasma membrane, resulting in ionic fluxes that, in turn, interfere with the normal formation of reservosomes.
在含有50 mM三磷酸腺苷（ATP）的培养基中孵育24小时会使克氏锥虫前鞭毛体的超微结构产生明显变化，其中最明显的是在细胞质中形成大的膜结合空泡。这些空泡在暴露于大分子辣根过氧化物酶（HRP）后呈阳性。在磷酸盐缓冲盐水（PBS）中进行20分钟的追踪后，HRP阳性空泡开始分离成离散结构，以至于在60分钟的追踪后，可以识别出明显的贮存小体。据推测，细胞外ATP会导致前鞭毛体细胞膜通透性增加，从而导致离子通量变化，进而干扰贮存小体的正常形成。
