Rinaldi T, Lande R, Bolotin-Fukuhara M, Frontali L
Pasteur Institute-Cenci Bolognetti Foundation, Department of Cell and Developmental Biology, University of Rome I, I-00185 Rome, Italy.
Curr Genet. 1997 Jun;31(6):494-6. doi: 10.1007/s002940050235.
In an attempt to identify new nuclear genes involved in the synthesis and processing of mitochondrial tRNAs, we utilized a multicopy nuclear library to suppress the heat-sensitive phenotype of a Saccharomyces cerevisiae mitochondrial mutant strain. This strain (Ts 932) is defective in the 3'-end processing of the mitochondrial tRNAAsp transcript. The nuclear genes coding for the mitochondrial elongation factor Tuf M and for the mitochondrial aspartyl-tRNA synthetase have been found to restore the temperature-resistant phenotype and to correct the RNA processing defect. Suppression was effective even when the genes were present on a centromeric plasmid.
为了鉴定参与线粒体tRNA合成和加工的新核基因，我们利用一个多拷贝核文库来抑制酿酒酵母线粒体突变菌株的热敏感表型。该菌株（Ts 932）在线粒体tRNAAsp转录本的3'末端加工方面存在缺陷。已发现编码线粒体延伸因子Tuf M和线粒体天冬氨酰-tRNA合成酶的核基因可恢复耐温表型并纠正RNA加工缺陷。即使这些基因存在于着丝粒质粒上，抑制也是有效的。
