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酵母线粒体tRNA突变体的核和线粒体回复突变体

Nuclear and mitochondrial revertants of a yeast mitochondrial tRNA mutant.

作者信息

Kang Y W, Miller D L

机构信息

Biology Programs, University of Texas at Dallas, Richardson 75083-0688.

出版信息

Mol Gen Genet. 1988 Aug;213(2-3):425-34. doi: 10.1007/BF00339612.

DOI:10.1007/BF00339612
PMID:3054486
Abstract

We isolated revertants capable of respiration from the respiratory deficient yeast mutant, FF1210-6C/170, which displays greatly decreased mitochondrial protein synthesis due to a single base substitution at the penultimate base of the tRNAAsp gene on mitochondrial (mt) DNA. Three classical types of revertant were identified: (1) same-site revertants; (2) intragenic revertants which restore the base pairing in the acceptor stem of the mitochondrial tRNAAsp; and (3) extragenic suppressors located in nuclear DNA. In addition a fourth type of revertant was identified in which the mutant tRNAAsp is amplified due to the maintenance of both the original mutant mtDNA and a modified form of the mutant mtDNA in which only a small region around the tRNAAsp gene is retained and amplified. The latter form resembles the mtDNA in vegetative petite (rho-) strains which normally segregates rapidly from the wild-type mtDNA. Each revertant type was characterized genetically and by both DNA sequence analysis of the mitochondrial tRNAAsp gene and analysis of the quantity and size of RNA containing the tRNAAsp sequence. These results indicate that the mitochondrial tRNAAsp of the mutant retains a low level of activity and that the presence of the terminal base pair in tRNAAsp is a determinant of both tRNAAsp function and the maintenance of wild-type levels of tRNAAsp.

摘要

我们从呼吸缺陷型酵母突变体FF1210 - 6C/170中分离出了能够进行呼吸作用的回复突变体。该突变体由于线粒体（mt）DNA上天冬氨酸tRNA（tRNAAsp）基因倒数第二个碱基处的单个碱基替换，导致线粒体蛋白质合成大幅减少。我们鉴定出了三种经典类型的回复突变体：（1）同一位点回复突变体；（2）恢复线粒体tRNAAsp接受臂碱基配对的基因内回复突变体；（3）位于核DNA中的基因外抑制子。此外，还鉴定出了第四种类型的回复突变体，其中突变的tRNAAsp由于原始突变型mtDNA和突变型mtDNA的一种修饰形式的同时存在而被扩增，在这种修饰形式中，仅tRNAAsp基因周围的一小区域被保留并扩增。后一种形式类似于营养型小菌落（rho-）菌株中的mtDNA，其通常会迅速从野生型mtDNA中分离出来。每种回复突变体类型都通过遗传学方法进行了表征，并对线粒体tRNAAsp基因进行了DNA序列分析，同时分析了含有tRNAAsp序列的RNA的数量和大小。这些结果表明，突变体的线粒体tRNAAsp保留了低水平的活性，并且tRNAAsp中末端碱基对的存在是tRNAAsp功能以及野生型tRNAAsp水平维持的一个决定因素。

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引用本文的文献

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A missense mutation in the nuclear gene coding for the mitochondrial aspartyl-tRNA synthetase suppresses a mitochondrial tRNA(Asp) mutation.编码线粒体天冬氨酰 - tRNA合成酶的核基因中的错义突变抑制了线粒体tRNA（Asp）突变。
Nucleic Acids Res. 2000 Apr 1;28(7):1542-7. doi: 10.1093/nar/28.7.1542.
2
Genetic instability of an oligomycin resistance mutation in yeast is associated with an amplification of a mitochondrial DNA segment.
Nucleic Acids Res. 1989 Sep 12;17(17):6927-37. doi: 10.1093/nar/17.17.6927.
3
Structure and transcription of the mitochondrial genome in heteroplasmic strains of Saccharomyces cerevisiae.酿酒酵母异质性菌株中线粒体基因组的结构与转录

本文引用的文献

1
Respiration deficient mutants in the A+T-rich region on yeast mitochondrial DNA containing the var1 gene.酵母线粒体 DNA 上 A+T 丰富区含有 var1 基因的呼吸缺陷突变体。
Curr Genet. 1982 Dec;6(3):179-88. doi: 10.1007/BF00390336.
2
A mutation in the tRNAAsp gene from yeast mitochondria. Effects on RNA and protein synthesis.酵母线粒体中天冬氨酸转运RNA基因的突变。对RNA和蛋白质合成的影响。
J Biol Chem. 1981 Oct 10;256(19):9774-7.
3
Rapid purification of yeast mitochondrial DNA in high yield.高产快速纯化酵母线粒体DNA
Nucleic Acids Res. 1989 Nov 11;17(21):8595-609. doi: 10.1093/nar/17.21.8595.
Biochim Biophys Acta. 1980 Dec 11;610(2):221-8. doi: 10.1016/0005-2787(80)90003-9.
4
Assembly of the mitochondrial membrane system. CBP1, a yeast nuclear gene involved in 5' end processing of cytochrome b pre-mRNA.线粒体膜系统的组装。CBP1，一个参与细胞色素b前体mRNA 5'端加工的酵母核基因。
J Biol Chem. 1984 Apr 25;259(8):4722-31.
5
Mitochondrial ribosomal RNA genes of yeast: their mutations and a common nuclear suppressor.酵母线粒体核糖体RNA基因：它们的突变及一种常见的核抑制因子。
Mol Gen Genet. 1984;193(2):275-9. doi: 10.1007/BF00330680.
6
Genetics and biogenesis of cytochrome b.细胞色素b的遗传学与生物合成
Methods Enzymol. 1983;97:374-95. doi: 10.1016/0076-6879(83)97150-1.
7
Identification and consequences of a guanosine-15 to adenosine-15 change in the yeast mitochondrial tRNASerUCX gene.酵母线粒体tRNASerUCX基因中鸟苷15位变为腺苷15位的鉴定及其后果
Biochemistry. 1983 Mar 29;22(7):1709-14. doi: 10.1021/bi00276a029.
8
A nuclear mutation that post-transcriptionally blocks accumulation of a yeast mitochondrial gene product can be suppressed by a mitochondrial gene rearrangement.一种转录后阻断酵母线粒体基因产物积累的核突变可被线粒体基因重排所抑制。
J Mol Biol. 1984 Jun 5;175(4):431-52. doi: 10.1016/0022-2836(84)90178-5.
9
Origins of transcripts of the yeast mitochondrial var 1 gene.酵母线粒体var 1基因转录本的起源
J Biol Chem. 1984 May 10;259(9):6019-27.
10
oli1 Transcripts in wild type and in a cytoplasmic "petite" mutant of yeast.酵母野生型和细胞质“小菌落”突变体中的oli1转录本。
J Biol Chem. 1983 Dec 10;258(23):14065-8.