Legeais J M, Drubaix I, Briat B, Savoldelli M, Ménasche M, Robert L, Renard G, Pouliquen Y
Département d'Ophtalmologie Hôtel-Dieu de Paris, INSERM U 86, France.
J Biomed Mater Res. 1997 Jul;36(1):49-54. doi: 10.1002/(sici)1097-4636(199707)36:1<49::aid-jbm6>3.0.co;2-l.
Microporous polymers have great potential for the production of corneal keratoprosthetic devices. Keratocytes invade the pores of expanded polytetrafluoroethylene implants (ePTFE) and collagen synthesis occurs. This ePTFE becomes translucent after its implantation in the stroma of rabbit cornea. The rate and density of cell growth within this polymer depends on the implant thickness, pore size, and its placement in the cornea. We have investigated the influence of the polymer permeability on the collagen and protein contents ePTFE implants. Rabbit corneal stroma were implanted with ePTFE disks (6 mm in diameter) by intralamellar keratoplasty. The implanted polymers were removed from the stroma after 3 to 6 months. The collagen and protein contents were determined after pepsin solubilization. The collagen content of the high-permeability implant was 3.7-fold greater than that of the low-permeability implant 3 months after implantation and 2.4-fold greater after 6 months. The total protein content of the high-permeability implant was 2.5-fold greater than that of low-permeability implant at 3 months and was the same after 6 months. The collagen-to-protein ratio was 68% in the high-permeability implants, and thus similar to that of normal corneal stroma. Thus, high polymer permeability increased both the rate and density of the corneal extracellular matrix ingrowth.
微孔聚合物在角膜角膜假体装置的生产中具有巨大潜力。角膜细胞侵入膨体聚四氟乙烯植入物（ePTFE）的孔隙并发生胶原合成。这种ePTFE植入兔角膜基质后会变得半透明。该聚合物内细胞生长的速率和密度取决于植入物的厚度、孔径及其在角膜中的位置。我们研究了聚合物渗透性对ePTFE植入物中胶原和蛋白质含量的影响。通过板层角膜移植术将ePTFE盘（直径6毫米）植入兔角膜基质。3至6个月后从基质中取出植入的聚合物。胃蛋白酶溶解后测定胶原和蛋白质含量。植入后3个月，高渗透性植入物的胶原含量比低渗透性植入物高3.7倍，6个月后高2.4倍。高渗透性植入物的总蛋白质含量在3个月时比低渗透性植入物高2.5倍，6个月后相同。高渗透性植入物中胶原与蛋白质的比率为68%，因此与正常角膜基质相似。因此，高聚合物渗透性增加了角膜细胞外基质向内生长的速率和密度。
