Drubaix I, Legeais J M, Malek-Chehire N, Savoldelli M, Ménasche M, Robert L, Renard G, Pouliquen Y
INSERM U86, Laboratoire de Biochimie de la Cornée, Paris, France.
Exp Eye Res. 1996 Apr;62(4):367-76. doi: 10.1006/exer.1996.0042.
The integration of microporous polymer into tissues is of great interest for the production of keratoprosthetic devices. Our previous studies showed functional differentiated cells and collagen synthesis in the pore of an expanded polytetrafluoroethylene implant. This study identifies and quantifies collagen types synthesized in the implant. Expanded polytetrafluoroethylene polymers were implanted in the rabbit corneas. The collagen extracted from the polymer and implanted stroma after 1, 3 and 6 months was quantified by measuring hydroxyproline. The relative proportions of collagen types were determined by densitometric analysis after SDS-PAGE. The collagen-to-protein ratio in the polymer increased from 0.22 to 0.70 between the first and third month after implantation becoming similar to control cornea. So that of the protein and collagen densities in the polymer and implanted stroma were similar to the control from the third month. The collagen synthesized in the polymer was mainly type I (87%) plus a small amount of type III (8%) 1 month after implantation. The collagen distribution from the third month after implantation was similar to that of the controls and remained constant thereafter in the polymer implant and in the implanted stroma. Immunogold labelling techniques confirmed these results. Implantation of this PTFE disc induced no obvious modification of the corneal stroma, confirming that this polymer is a good interface that is compatible with the native corneal stroma. The keratocytes in this polymer rapidly adopted a corneal phenotype, distinct from the dermal or scaring phenotype as shown by the collagen types produced in the implant.
将微孔聚合物整合到组织中对于角膜假体装置的生产具有极大的意义。我们之前的研究表明，在膨体聚四氟乙烯植入物的孔隙中存在功能分化的细胞和胶原蛋白合成。本研究鉴定并定量了植入物中合成的胶原蛋白类型。将膨体聚四氟乙烯聚合物植入兔角膜。通过测量羟脯氨酸对植入后1个月、3个月和6个月从聚合物和植入基质中提取的胶原蛋白进行定量。通过SDS-PAGE后的光密度分析确定胶原蛋白类型的相对比例。植入后第一个月到第三个月之间，聚合物中的胶原蛋白与蛋白质的比例从0.22增加到0.70，变得与对照角膜相似。因此，从第三个月起，聚合物和植入基质中的蛋白质和胶原蛋白密度与对照相似。植入后1个月，聚合物中合成的胶原蛋白主要是I型（87%）加上少量III型（8%）。植入后第三个月起，聚合物植入物和植入基质中的胶原蛋白分布与对照相似，此后保持不变。免疫金标记技术证实了这些结果。植入这种聚四氟乙烯盘对角膜基质没有明显影响，证实这种聚合物是与天然角膜基质相容的良好界面。该聚合物中的角膜细胞迅速呈现角膜表型，与真皮或瘢痕表型不同，这从植入物中产生的胶原蛋白类型可以看出。
