几种红球菌中质粒编码的二苯并噻吩脱硫基因的保守性
Conservation of plasmid-encoded dibenzothiophene desulfurization genes in several rhodococci.
作者信息
Denis-Larose C, Labbé D, Bergeron H, Jones A M, Greer C W, al-Hawari J, Grossman M J, Sankey B M, Lau P C
机构信息
Biotechnology Research Institute, National Research Council Canada, Montreal, Quebec, Canada.
出版信息
Appl Environ Microbiol. 1997 Jul;63(7):2915-9. doi: 10.1128/aem.63.7.2915-2919.1997.
Abstract
The cloned sulfur oxidation (desulfurization) genes (sox) for dibenzothiophene (DBT) from the prototype Rhodococcus sp. strain IGTS8 were used in Southern hybridization and PCR experiments to establish the DNA relatedness in six new rhodococcal isolates which are capable of utilizing DBT as a sole sulfur source for growth. The ability of these strains to desulfurize appears to be an exclusive property of a 4-kb gene locus on a large plasmid of ca. 150 kb in IGTS8 and ca. 100 kb in the other strains. Besides a difference in plasmid profile, IGTS8 is distinguishable from the other strains in at least the copy number of the insertion sequence IS1166, which is associated with the sox genes.
摘要
来自原型红球菌属菌株IGTS8的用于二苯并噻吩（DBT）的克隆硫氧化（脱硫）基因（sox）被用于Southern杂交和PCR实验，以确定六种新的红球菌分离株中的DNA相关性，这些分离株能够利用DBT作为唯一硫源进行生长。这些菌株的脱硫能力似乎是IGTS8中约150 kb大质粒上一个4 kb基因座的独特特性，而在其他菌株中该质粒约为100 kb。除了质粒图谱存在差异外，IGTS8与其他菌株的区别至少还在于与sox基因相关的插入序列IS1166的拷贝数。
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