A new indirect spectrofluorimetric determination of sub-micromolar concentrations of manganese(II) in bovine liver by means of 2-hydroxyquinoline.

A new high-sensitive and at the same time accurate and well documented indirect spectrofluorimetric method for the determination of sub-micromolar concentrations of manganese(II) being as a normal trace element in the bovine liver, was described. The purpose of the experimental work is performed by means of two fluorescence intensity measurements; the measurement of the fluorescence (F1) of a 2.0 x 10(-5) mol/l solution of pure 2-hydroxyquinoline (2-HQ) (lambda ex: 320 nm: lambda em: 375 nm), and that of the fluorescence of the above solution after the addition of Mn(II) to be analyzed (F2). The observed difference of the fluorescence intensities measured (F1-F2) = delta F, is exclusively owed to the quenching of the Mn(II) on the free 2-HQ. The presence of various metal ions considerably interferes with the above described determination of Mn(II). For this reason, the separation and/or masking of these undesirable metal ions it is advisable to apply before any analytical praxis.