[锰（II）与EHPG结合的光谱研究]

[Spectroscopic study on the binding of Mn(II) to EHPG].

作者信息

Li Hai-peng, Zhao Chun-gui, Li Xiao-li, Yang Bin-sheng

机构信息

Key Lab of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2007 Feb;27(2):313-6.

PMID:17514964

Abstract

Under the conditions of 0.05 mol x L(-1) Hepes buffer at room temperature and pH 7.4, the interaction of ethylene-N,N'-bis(o-hydroxyphenylglycine) (EHPG) and Mn(II) was investigated by both fluorescence and UV difference spectra. Results showed that the molar ratio of the complex is 1:1. With the addition of manganese ions, the fluorescence peak of EHPG at 310 nm decreased, while the peaks of UV absorptivity at 238 and 291 nm increased. The molar absorptivity of Mn(II) to EHPG at 238 nm is (1.31 +/- 0.02) x 10(4) cm(-1) x mol(-1) L. The disassociation constant for Mn-EHPG was determined to be (1.36 +/- 0.21) x 10(-5). It can be concluded that the binding of Mn(II) to EHPG is not a strongly binding reaction.

摘要

在室温下、pH值为7.4的0.05 mol·L⁻¹ Hepes缓冲液条件下，通过荧光光谱和紫外差示光谱研究了乙二胺-N,N'-双（邻羟基苯甘氨酸）（EHPG）与锰（II）的相互作用。结果表明，配合物的摩尔比为1:1。随着锰离子的加入，EHPG在310 nm处的荧光峰降低，而在238和291 nm处的紫外吸光度峰增加。锰（II）在238 nm处对EHPG的摩尔吸光系数为(1.31±0.02)×10⁴ cm⁻¹·mol⁻¹·L。锰-EHPG的解离常数测定为(1.36±0.21)×10⁻⁵。可以得出结论，锰（II）与EHPG的结合不是强结合反应。