人白血病白细胞和L1210细胞中6-巯基嘌呤代谢的比较研究。
Comparative study of 6-mercaptopurine metabolism in human leukemic leukocytes and L1210 cells.
作者信息
Higuchi T, Nakamura T, Uchino H, Wakisaka G
出版信息
Antimicrob Agents Chemother. 1977 Oct;12(4):518-22. doi: 10.1128/AAC.12.4.518.
Leukocytes from patients with leukemia and L1210 cells from mice were examined for the rate of formation and cellular concentration of phosphoribosylpyrophosphate, the rate of thioinosinic acid formation, and a number of selected enzymes involved in purine nucleotide synthesis. The amount of thioinosinic acid formed in L1210 cells was much higher than that in human leukemic leukocytes. In cell extracts, the synthesis of thioinosinic acid was similar in both cell types, and the amount of purine phosphoribosyltransferase was not rate limiting in either case. Much higher concentrations and rates of formation of phosphoribosylpyrophosphate were found in L1210 cells than in human leukemic leukocytes. The difference in response to 6-mercaptopurine between L1210 cells and human leukemic leukocytes might be attributed to their difference in supply of phosphoribosylpyrophosphate. Phosphoribosylpyrophosphate-amidotransferase was found to be high in L1210 cells, but was not detected in human leukemic leukocytes.
对白血病患者的白细胞以及小鼠的L1210细胞进行了检测，以观察磷酸核糖焦磷酸的生成速率和细胞浓度、硫代次黄苷酸的生成速率以及嘌呤核苷酸合成过程中一些选定的酶。L1210细胞中形成的硫代次黄苷酸量远高于人类白血病白细胞中的量。在细胞提取物中，两种细胞类型中硫代次黄苷酸的合成相似，并且在两种情况下嘌呤磷酸核糖转移酶的量都不是限速因素。发现L1210细胞中磷酸核糖焦磷酸的浓度和生成速率远高于人类白血病白细胞。L1210细胞和人类白血病白细胞对6-巯基嘌呤反应的差异可能归因于它们在磷酸核糖焦磷酸供应上的差异。发现磷酸核糖焦磷酸酰胺转移酶在L1210细胞中含量较高，但在人类白血病白细胞中未检测到。
Zotero 插件