The AgNoR count in ulcerative colitis with and without dysplasia.

The numbers of specific intranuclear AgNOR silver precipitates (AgNORs) and their relation to the Inflammatory Bowel Disease-Dysplasia Morphology Study Group's classification were studied in 134 colonscopic biopsy specimens of ulcerative colitis (UC) with and without dysplastic findings from 73 patients with active and inactive disease. In addition, 40 cases of colonic adenocarcinomas and 22 normal colonic mucosae were also assessed for comparison with the one-step silver staining AgNOR technique. Statistical comparison of the mean AgNOR number revealed a highly significant inter-class difference (P < 0.001) between UC without dysplasia and UC with dysplasia as well as between active and inactive UC. On the contrary, the AgNORs count did not separate colonic adenocarcinomas from UC with dysplasia either high or low grade nor these three classes from the group of UC with indefinite dysplasia. The quantitative evaluation of epithelial changes occurring in UC by the AgNOR method may help to establish the clinical management of colitis patients, in addition to qualitative morphological criteria.