降钙素基因相关肽（8-37）（一种胰淀素和降钙素基因相关肽拮抗剂）对糖尿病大鼠和正常大鼠胆囊收缩素及蛙皮素厌食作用的不同影响。

Different influence of CGRP (8-37), an amylin and CGRP antagonist, on the anorectic effects of cholecystokinin and bombesin in diabetic and normal rats.

作者信息

Lutz T A, Pieber T R, Walzer B, Del Prete E, Scharrer E

机构信息

Institute of Veterinary Physiology, University of Zuerich, Switzerland.

出版信息

Peptides. 1997;18(5):643-9. doi: 10.1016/s0196-9781(97)00124-1.

DOI:10.1016/s0196-9781(97)00124-1

PMID:9213356

Abstract

Because previous studies had suggested that the anorectic effects of cholecystokinin (CCK) and bombesin (BBS) depend partly on the release of amylin or calcitonin gene-related peptide (CGRP), we investigated the influence of the amylin and CGRP receptor antagonist CGRP (8-37) on the anorectic effects of CCK and BBS in streptozotocin (STZ)-diabetic and nondiabetic rats. STZ-diabetic rats had significantly lower plasma amylin and insulin concentrations than nondiabetic control rats. Amylin (5 micrograms/kg or 2.5 micrograms/rat) injected IP at dark onset after 24-h food deprivation elicited an anorectic effect of similar extent in STZ-diabetic and control rats. Under similar conditions, CCK (0.25 and 2 micrograms/kg) and BBS (5 micrograms/kg) reduced food intake in both STZ-diabetic and nondiabetic rats. These effects were markedly attenuated by CGRP (8-37) (10 micrograms/kg) in non-diabetics but not in STZ-diabetic rats. It is concluded that part of the anorectic effects of CCK and BBS depend on the release of amylin from pancreatic B-cells.

摘要

由于先前的研究表明胆囊收缩素（CCK）和蛙皮素（BBS）的厌食作用部分取决于胰淀素或降钙素基因相关肽（CGRP）的释放，我们研究了胰淀素和CGRP受体拮抗剂CGRP（8-37）对链脲佐菌素（STZ）诱导的糖尿病大鼠和非糖尿病大鼠中CCK和BBS厌食作用的影响。STZ诱导的糖尿病大鼠的血浆胰淀素和胰岛素浓度显著低于非糖尿病对照大鼠。在禁食24小时后于黑暗开始时腹腔注射胰淀素（5微克/千克或2.5微克/只大鼠），在STZ诱导的糖尿病大鼠和对照大鼠中引起了相似程度的厌食作用。在相似条件下，CCK（0.25和2微克/千克）和BBS（5微克/千克）减少了STZ诱导的糖尿病大鼠和非糖尿病大鼠的食物摄入量。在非糖尿病大鼠中，这些作用被CGRP（8-37）（10微克/千克）显著减弱，但在STZ诱导的糖尿病大鼠中则没有。结论是，CCK和BBS的部分厌食作用取决于胰腺β细胞中胰淀素的释放。