Westacott C I, Webb G R, Warnock M G, Sims J V, Elson C J
University of Bristol, UK.
Arthritis Rheum. 1997 Jul;40(7):1282-91. doi: 10.1002/1529-0131(199707)40:7<1282::AID-ART13>3.0.CO;2-E.
To determine whether bone cells alter cartilage metabolism.
Bone cell cultures were established using explants obtained from the hip and knee joints of 9 patients with osteoarthritis (OA) and 6 subjects without arthritis (nonarthritic [NA]). NA human cartilage biopsy samples were incubated in the presence or absence of bone-derived cells, and the effects on glycosaminoglycan (GAG) release from cartilage were measured.
Bone cell cultures secreted osteocalcin (OC) and did not contain cells expressing leukocyte common antigen. None of the 8 cultures established from NA bone, compared with 17 of 32 from OA bone, significantly altered GAG release from cartilage (P = 0.006). In knees with medial joint damage, 38% of the cultures derived from the medial side of the joint increased GAG release from cartilage. In contrast, 77% of the cultures derived from the lateral side of the joint had an effect on GAG, with 38% increasing and 38% decreasing GAG release. Seven cytokines were measured in OA bone cell supernatants. No significant difference was apparent in the concentration of any one cytokine when supernatants were compared according to their effects on GAG release.
Bone cells from OA patients can influence cartilage metabolism. This might explain why increased subchondral bone activity can predict cartilage loss.
确定骨细胞是否会改变软骨代谢。
使用从9例骨关节炎（OA）患者和6例无关节炎受试者（非关节炎[NA]）的髋关节和膝关节获取的外植体建立骨细胞培养物。将NA人软骨活检样本在有或无骨源细胞存在的情况下进行孵育，并测量其对软骨中糖胺聚糖（GAG）释放的影响。
骨细胞培养物分泌骨钙素（OC），且不含表达白细胞共同抗原的细胞。与32例OA骨来源的培养物中的17例相比，8例从NA骨建立的培养物中无一例显著改变软骨中GAG的释放（P = 0.006）。在有内侧关节损伤的膝关节中，38%来自关节内侧的培养物增加了软骨中GAG的释放。相比之下，77%来自关节外侧的培养物对GAG有影响，其中38%增加了GAG释放，38%降低了GAG释放。在OA骨细胞上清液中检测了七种细胞因子。根据上清液对GAG释放的影响进行比较时，任何一种细胞因子的浓度均无明显差异。
OA患者的骨细胞可影响软骨代谢。这可能解释了为什么软骨下骨活性增加可预测软骨丢失。
