Kondo H, Rabouille C, Newman R, Levine T P, Pappin D, Freemont P, Warren G
Cell Biology Laboratory, Imperial Cancer Research Fund, London, UK.
Nature. 1997 Jul 3;388(6637):75-8. doi: 10.1038/40411.
At least two distinct ATPases, NSF and p97, are known to be involved in the heterotypic fusion of transport vesicles with their target membranes and the homotypic fusion of membrane compartments. The NSF-mediated fusion pathway is the best characterized, many of the components having been identified and their functions analysed. In contrast, none of the accessory proteins for the p97-mediated fusion pathway has been identified. Now we have identified the first such component, a protein of relative molecular mass 47,000 (p47), which forms a tight, stoichiometric complex with cytosolic p97 (one trimer of p47 per hexamer of p97). It is essential for the p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments, a process restricted to animal cells. As a homologue of p47 exists in budding yeast, this indicates that it might also be involved in other membrane fusion reactions catalysed by p97, such as karyogamy.
已知至少有两种不同的ATP酶，即N-乙基马来酰胺敏感因子（NSF）和p97，参与运输小泡与其靶膜的异型融合以及膜区室的同型融合。NSF介导的融合途径的特征最为明确，许多组分已被鉴定，其功能也已得到分析。相比之下，p97介导的融合途径的辅助蛋白尚未被鉴定。现在我们鉴定出了第一个这样的组分，一种相对分子质量为47000的蛋白质（p47），它与胞质p97形成紧密的化学计量复合物（每六个p97亚基结合一个p47三聚体）。它对于有丝分裂后高尔基体片段中高尔基体潴泡的p97介导的再生至关重要，这一过程仅限于动物细胞。由于出芽酵母中存在p47的同源物，这表明它可能也参与p97催化的其他膜融合反应，如核融合。
