Syntaxin 5是体外有丝分裂高尔基体片段中NSF和p97介导的高尔基体池重新组装途径的常见组成部分。

Syntaxin 5 is a common component of the NSF- and p97-mediated reassembly pathways of Golgi cisternae from mitotic Golgi fragments in vitro.

作者信息

Rabouille C, Kondo H, Newman R, Hui N, Freemont P, Warren G

机构信息

Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.

出版信息

Cell. 1998 Mar 6;92(5):603-10. doi: 10.1016/s0092-8674(00)81128-9.

DOI:10.1016/s0092-8674(00)81128-9

PMID:9506515

Abstract

A cell-free system that mimics the reassembly of Golgi stacks at the end of mitosis requires two ATPases, NSF and p97, to rebuild Golgi cisternae. Morphological studies now show that alpha-SNAP, a component of the NSF pathway, can inhibit the p97 pathway, whereas p47, a component of the p97 pathway, can inhibit the NSF pathway. Anti-syntaxin 5 antibodies and a soluble, recombinant syntaxin 5 inhibited both pathways, suggesting that this t-SNARE is a common component. Biochemical studies confirmed this, showing that p47 binds directly to syntaxin 5 and competes for binding with alpha-SNAP. p47 also mediates the binding of p97 to syntaxin 5 and so plays an analogous role to alpha-SNAP, which mediates the binding of NSF.

摘要

一种模拟有丝分裂末期高尔基体堆叠重新组装的无细胞系统需要两种ATP酶，即NSF和p97，来重建高尔基体潴泡。形态学研究现表明，NSF途径的一个组分α-SNAP可抑制p97途径，而p97途径的一个组分p47可抑制NSF途径。抗Syntaxin 5抗体和一种可溶性重组Syntaxin 5抑制了这两种途径，提示这种t-SNARE是一个共同组分。生化研究证实了这一点，表明p47直接结合Syntaxin 5并竞争与α-SNAP的结合。p47还介导p97与Syntaxin 5的结合，因此发挥着与介导NSF结合的α-SNAP类似的作用。