Grune T, Michel P, Sitte N, Eggert W, Albrecht-Nebe H, Esterbauer H, Siems W G
Clinic of Physical Medicine and Rehabilitation, Medical Faculty (Charité), Humboldt University Berlin, Germany.
Free Radic Biol Med. 1997;23(3):357-60. doi: 10.1016/s0891-5849(96)00586-2.
Analysis of serum samples of healthy children (n = 11) and children with Systemic Lupus Erythematosus (SLE), (n = 21) was performed by SDS-PAGE and immunoblot with an antibody directed against proteins modified by lipid peroxidation (LPO) product 4-hydroxynonenal (HNE). A single major stained protein band was detected. By comparison of the molecular weights in nonreducing and reducing SDS-PAGE was found that the main protein modified by HNE is immunoglobulin G. Significantly higher concentrations of the aldehyde modified protein were found in children with high disease activity of SLE measured by SLE disease activity index (SLEDAI). Lipid peroxidation measured by malondialdehyde and 4-hydroxynonenal concentrations show an enhanced level of both compounds also in patients with the active autoimmune disease. Therefore, it can be assumed that free radical mediated processes play a pathophysiological role in the active phase of SLE and HNE-modified serum proteins are a further parameter for the detection of in vivo LPO.
采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹法，使用针对脂质过氧化（LPO）产物4-羟基壬烯醛（HNE）修饰蛋白的抗体，对11名健康儿童和21名系统性红斑狼疮（SLE）患儿的血清样本进行分析。检测到一条主要的染色蛋白条带。通过比较非还原和还原SDS-PAGE中的分子量，发现被HNE修饰的主要蛋白是免疫球蛋白G。通过SLE疾病活动指数（SLEDAI）测量，在疾病活动度高的SLE患儿中发现醛修饰蛋白的浓度显著更高。通过丙二醛和4-羟基壬烯醛浓度测量的脂质过氧化表明，在活动性自身免疫疾病患者中这两种化合物的水平也有所升高。因此，可以假设自由基介导的过程在SLE的活动期起病理生理作用，而HNE修饰的血清蛋白是体内LPO检测的另一个参数。
