Kavutcu M, Canbolat O, Oztürk S, Olcay E, Ulutepe S, Ekinci C, Gökhun I H, Durak I
Department of Biochemistry, Ankara University Medical Faculty, Turkiye.
Nephron. 1996;72(2):269-74. doi: 10.1159/000188853.
In this study, the activities of major enzymes participating in free radical metabolism (xanthine oxidase, XO; Cu,Zn and Mn superoxide dismutases, SOD; glutathione peroxidase, GSH-Px; catalase, CAT) were measured in kidney tissues from guinea pigs treated with gentamicin alone (200 mg/kg/day), gentamicin plus vitamin C (600 mg/kg/day), gentamicin plus vitamin E (400 mg/kg/day), and gentamicin plus vitamins C and E together for 10 days, and from animals treated with physiological saline solution alone during this period. We found no significant differences between control and gentamicin groups with respect to XO and Cu,Zn-SOD activities. However, the activities of Mn-SOD, GSH-Px, and CAT were found to be significantly depressed in the gentamicin-treated group relative to controls. In the gentamicin plus vitamin C group, the renal tissue Mn-SOD activity was found to be higher as compared with control and gentamicin groups. In this group, XO, GSH-Px and CAT activities were also higher than in the gentamicin-treated group, but no statistically significant differences existed between the values of this group and controls. Similar results were also observed in the gentamicin plus vitamin E group for Mn-SOD, GSH-Px, CAT, and XO. In this group, the Cu,Zn-SOD activity was found to be decreased as compared with control and gentamicin groups. In the gentamicin plus vitamins C and E group, the Cu,Zn-SOD activity was found to be decreased, the XO activity to be unchanged, and Mn-SOD, GSH-Px, and CAT activities to be increased as compared with the gentamicin and control groups. The results suggest that the enzymatic antioxidant defense system was significantly disturbed because of the suppressed activities of Mn-SOD, GSH-Px, and CAT in the kidney tissues from animals treated with gentamicin. However, vitamins C and E given concurrently with gentamicin completely abrogated this enzymatic suppression.
在本研究中，对单独用庆大霉素（200毫克/千克/天）、庆大霉素加维生素C（600毫克/千克/天）、庆大霉素加维生素E（400毫克/千克/天）以及庆大霉素加维生素C和E联合处理10天的豚鼠肾脏组织，和在此期间单独用生理盐水处理的动物肾脏组织，测定了参与自由基代谢的主要酶（黄嘌呤氧化酶，XO；铜锌和锰超氧化物歧化酶，SOD；谷胱甘肽过氧化物酶，GSH-Px；过氧化氢酶，CAT）的活性。我们发现，在XO和铜锌超氧化物歧化酶活性方面，对照组和庆大霉素组之间没有显著差异。然而，相对于对照组，庆大霉素处理组中锰超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶的活性显著降低。在庆大霉素加维生素C组中，发现肾脏组织锰超氧化物歧化酶活性高于对照组和庆大霉素组。在该组中，XO、谷胱甘肽过氧化物酶和过氧化氢酶的活性也高于庆大霉素处理组，但该组与对照组之间的值没有统计学上的显著差异。在庆大霉素加维生素E组中，锰超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶和XO也观察到类似结果。在该组中，发现铜锌超氧化物歧化酶活性相对于对照组和庆大霉素组有所降低。在庆大霉素加维生素C和E组中，发现铜锌超氧化物歧化酶活性降低，XO活性不变，而锰超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶活性相对于庆大霉素组和对照组有所增加。结果表明，由于用庆大霉素处理的动物肾脏组织中锰超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶的活性受到抑制，酶促抗氧化防御系统受到显著干扰。然而，与庆大霉素同时给予的维生素C和E完全消除了这种酶抑制作用。
