超临界二氧化碳微泡法对芽孢杆菌孢子的灭活作用
Inactivation of Bacillus spores by the supercritical carbon dioxide micro-bubble method.
作者信息
Ishikawa H, Shimoda M, Tamaya K, Yonekura A, Kawano T, Osajima Y
机构信息
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
出版信息
Biosci Biotechnol Biochem. 1997 Jun;61(6):1022-3. doi: 10.1271/bbb.61.1022.
Bacillus spores were effectively inactivated by the supercritical (SC) CO2 micro-bubble method. The micro-bubble SC CO2 treatment of B. cereus, B. subtilis, B. megaterium, B. polymyxa, and B. coagulans at 40 degrees C and 30 MPa for 30 min produced greater reduction (about 3 log cycles of reduction) than a similar treatment without a filter. The SC CO2 treatment of B. polymyxa, B. cereus, and B. subtilis spores at 45 degrees C, 50 degrees C, respectively, and 30 MPa for 60 min resulted in a 6-log cycle reduction of survival. The SC CO2 treatment under the foregoing conditions should offer higher efficiency than that of heat treatment at 100 degrees C for 60 min. In addition, the SC CO2 treatment (30 MPa, 60 degrees C, 30 min) of B. polymyxa and B. cereus spores also produced a 6-log cycle reduction.
芽孢杆菌孢子可通过超临界（SC）二氧化碳微泡法有效灭活。在40℃和30MPa下对蜡状芽孢杆菌、枯草芽孢杆菌、巨大芽孢杆菌、多粘芽孢杆菌和凝结芽孢杆菌进行30分钟的微泡SC二氧化碳处理，与未使用过滤器的类似处理相比，产生了更大程度的减少（约3个对数循环的减少）。分别在45℃、50℃和30MPa下对多粘芽孢杆菌、蜡状芽孢杆菌和枯草芽孢杆菌孢子进行60分钟的SC二氧化碳处理，导致存活率降低6个对数循环。上述条件下的SC二氧化碳处理应比在100℃下热处理60分钟具有更高的效率。此外，对多粘芽孢杆菌和蜡状芽孢杆菌孢子进行SC二氧化碳处理（30MPa，60℃，30分钟）也导致6个对数循环的减少。
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