Titration of human placental alkaline phosphatase with radioactive orthophosphate.
作者信息
Whitaker K B, Moss D W
出版信息
Clin Chim Acta. 1976 Sep 6;71(2):277-84. doi: 10.1016/0009-8981(76)90541-6.
PMID:9215
Abstract
Human placental alkaline phosphatase incorporates radioactive phosphate specifically and covalently at acid pH. By titration of solutions of the purified enzyme with radioactive orthophosphate, the enzyme was shown to incorporate up to 2 phosphate groups per molecule. No evidence was found to suggest that the two sites had different affinities for phosphate. Similar titrations can be used to determine the molarity of solutions of non-placental alkaline phosphatases of unknown purity, if these also are assumed to possess 2 binding sites per molecule.
摘要
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Comparison of radioactive peptides obtained from specifically labelled human renal and placental alkaline phosphatases.从特异性标记的人肾和胎盘碱性磷酸酶获得的放射性肽的比较。
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