从特异性标记的人肾和胎盘碱性磷酸酶获得的放射性肽的比较。
Comparison of radioactive peptides obtained from specifically labelled human renal and placental alkaline phosphatases.
作者信息
Whitaker K B, Moss D W
出版信息
Biochem J. 1979 Oct 1;183(1):189-92. doi: 10.1042/bj1830189.
Abstract
Peptides with different chromatographic and electrophoretic properties were obtained from human placental and renal alkaline phosphatases by tryptic digestion of the enzymes labelled with radioactive orthophosphate at their active centres. These results provide structural evidence for the distinct genetic origins of the two isoenzymes that had previously been inferred from their different properties and from the observed phenotypic variation of placental phosphatase.
摘要
通过对在活性中心用放射性正磷酸盐标记的人胎盘碱性磷酸酶和肾碱性磷酸酶进行胰蛋白酶消化，获得了具有不同色谱和电泳特性的肽段。这些结果为两种同工酶不同的遗传起源提供了结构证据，这两种同工酶先前已根据它们不同的特性以及观察到的胎盘磷酸酶表型变异推断得出。
相似文献
1
Comparison of radioactive peptides obtained from specifically labelled human renal and placental alkaline phosphatases.从特异性标记的人肾和胎盘碱性磷酸酶获得的放射性肽的比较。
Biochem J. 1979 Oct 1;183(1):189-92. doi: 10.1042/bj1830189.
2
Comparison of human alkaline phosphatase isoenzymes. Structural evidence for three protein classes.人碱性磷酸酶同工酶的比较。三类蛋白质的结构证据。
Biochem J. 1979 Jul 1;181(1):67-73. doi: 10.1042/bj1810067.
3
An active site peptide from human placental alkaline phosphatase.人胎盘碱性磷酸酶的一个活性位点肽段。
Clin Chim Acta. 1976 Sep 6;71(2):285-91. doi: 10.1016/0009-8981(76)90542-8.
4
Titration of human placental alkaline phosphatase with radioactive orthophosphate.
Clin Chim Acta. 1976 Sep 6;71(2):277-84. doi: 10.1016/0009-8981(76)90541-6.
5
Different genes code for alkaline phosphatases from human fetal and adult intestine.
Biochem Biophys Res Commun. 1985 Jan 16;126(1):427-33. doi: 10.1016/0006-291x(85)90623-0.
6
An investigation into the immunochemical properties of the isoenzymes of human alkaline phosphatase.人碱性磷酸酶同工酶免疫化学性质的研究。
Ann Clin Biochem. 1977 Jul;14(4):212-7. doi: 10.1177/000456327701400158.
7
Evidence that three structural genes code for human alkaline phosphatases.有证据表明三个结构基因编码人类碱性磷酸酶。
Nature. 1979 Sep 13;281(5727):152-4. doi: 10.1038/281152a0.
8
Monoclonal antibodies block the trypsin cleavage site on human placental alkaline phosphatase.
FEBS Lett. 1984 Aug 6;173(2):357-9. doi: 10.1016/0014-5793(84)80805-4.
9
Structural evidence that human liver and placental alkaline phosphatase isoenzymes are coded by different genes.
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2201-5. doi: 10.1073/pnas.73.7.2201.
10
Synthesis and secretion of alkaline phosphatase in vitro from first-trimester and term human placentas.孕早期和足月人胎盘碱性磷酸酶的体外合成与分泌
Biochem J. 1981 Mar 15;194(3):857-66. doi: 10.1042/bj1940857.
引用本文的文献
1
Characterization of human foetal intestinal alkaline phosphatase. Comparison with the isoenzymes from the adult intestine and human tumour cell lines.人胎儿肠碱性磷酸酶的特性。与成人肠道和人肿瘤细胞系的同工酶比较。
Biochem J. 1983 Jun 1;211(3):553-8. doi: 10.1042/bj2110553.
本文引用的文献
1
THE AMINO ACID SEQUENCE OF A TETRADECAPEPTIDE CONTAINING THE REACTIVE SERINE IN E. COLI ALKALINE PHOSPHATASE.大肠杆菌碱性磷酸酶中含活性丝氨酸的十四肽的氨基酸序列。
Proc Natl Acad Sci U S A. 1963 May;49(5):722-9. doi: 10.1073/pnas.49.5.722.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
THE AMINO ACID SEQUENCE AROUND THE REACTIVE SERINE IN CALF-INTESTINAL ALKALINE PHOSPHATASE.小牛肠碱性磷酸酶中活性丝氨酸周围的氨基酸序列。
Biochim Biophys Acta. 1964 Oct 23;92:79-84.
4
Human organ alkaline phosphatases: discrimination by several means including starch gel electrophoresis of antienzyme-enzyme supernatant fluids.人体器官碱性磷酸酶：通过多种方法进行鉴别，包括对抗酶-酶上清液进行淀粉凝胶电泳。
Ann N Y Acad Sci. 1963 May 8;103:938-51. doi: 10.1111/j.1749-6632.1963.tb53746.x.
5
Phosphate incorporation into alkaline phosphatase of E. coli.磷酸盐掺入大肠杆菌碱性磷酸酶中。
Proc Natl Acad Sci U S A. 1961 Dec 15;47(12):1996-2005. doi: 10.1073/pnas.47.12.1996.
6
Studies on calf-intestinal alkaline phosphatase. II. Incorporation of inorganic phosphate into a highly purified enzyme preparation.小牛肠碱性磷酸酶的研究。II. 无机磷酸盐掺入高度纯化的酶制剂中。
Biochim Biophys Acta. 1961 Sep 2;52:49-59. doi: 10.1016/0006-3002(61)90902-7.
7
The catalytic-centre activity and kinetic properties of bovine milk alkaline phosphatase.牛乳碱性磷酸酶的催化中心活性及动力学特性
Biochem J. 1966 Nov;101(2):460-6. doi: 10.1042/bj1010460.
8
Studies on human placental alkaline phosphatase. I. Purification and crystallization.人胎盘碱性磷酸酶的研究。I. 纯化与结晶。
Arch Biochem Biophys. 1968 Aug;126(2):503-12. doi: 10.1016/0003-9861(68)90435-9.
9
Partial purification and some properties of human bone alkaline phosphatase.
Enzymologia. 1968 Jul 31;35(1):31-9.
10
An estimate of the catalytic-centre activity and purity of human alkaline phosphatase preparations.
Biochim Biophys Acta. 1968 Apr 9;154(3):609-12. doi: 10.1016/0005-2795(68)90028-7.
Zotero 插件