Bachert C, Wagenmann M, Hauser U, Rudack C
Ear, Nose, and Throat Department, University of Düsseldorf.
J Allergy Clin Immunol. 1997 Jun;99(6 Pt 1):837-42. doi: 10.1016/s0091-6749(97)80019-x.
In most nasal polyps, tissue eosinophilia is a striking finding, the pathologic mechanism of which is not understood.
This study was performed to investigate a possibly distinct cytokine and chemokine pattern that could explain the characteristic tissue eosinophilia in nasal polyps.
Polyps from 23 patients and turbinate tissue from 18 control subjects were investigated. The cytokine protein content (IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-8, IL-10, tumor necrosis factor-alpha, granulocyte-macrophage colony-stimulating factor, IL-1RA, RANTES, GRO-alpha) of tissue homogenates was measured by ELISA. Immunohistochemistry was performed in selected samples to detect IL-5+, major basic protein-positive, and EG2+ cells.
IL-5 was detectable in only one sample of tissue from 18 control subjects but was found in 18 of 23 nasal polyps. Immunohistochemistry revealed an abundant number of IL-5+ cells, of which 69.5% could be identified as eosinophils by morphology. IL-6, IL-8, IL-10, tumor necrosis factor-alpha, GRO-alpha, and RANTES were detected in all specimens, without significant differences between groups (p > or = 0.05), whereas significnatly higher concentrations of IL-1 beta and IL-1RA were found in turbinate mucosa (p < or = 0.05). IL-3 was not detectable: granulocyte-macrophage colony-stimulating factor could only occasionally be found.
This study indicates that IL-5 plays a key role in the pathophysiology of eosinophilic nasal polyps and may be produced by eosinophils.
在大多数鼻息肉中，组织嗜酸性粒细胞增多是一个显著特征，但其病理机制尚不清楚。
本研究旨在探讨一种可能独特的细胞因子和趋化因子模式，以解释鼻息肉中特征性的组织嗜酸性粒细胞增多现象。
对23例患者的息肉组织和18例对照受试者的鼻甲组织进行研究。通过酶联免疫吸附测定法（ELISA）检测组织匀浆中的细胞因子蛋白含量（白细胞介素-1β、白细胞介素-3、白细胞介素-4、白细胞介素-5、白细胞介素-6、白细胞介素-8、白细胞介素-10、肿瘤坏死因子-α、粒细胞-巨噬细胞集落刺激因子、白细胞介素-1受体拮抗剂、调节激活正常T细胞表达和分泌因子、生长调节致癌基因-α）。对选定样本进行免疫组织化学检测，以检测白细胞介素-5阳性、主要碱性蛋白阳性和嗜酸性粒细胞阳离子蛋白阳性细胞。
在18例对照受试者的组织样本中，仅在1个样本中检测到白细胞介素-5，但在23例鼻息肉中的18例中发现了白细胞介素-5。免疫组织化学显示有大量白细胞介素-5阳性细胞，其中69.5%在形态上可鉴定为嗜酸性粒细胞。在所有标本中均检测到白细胞介素-6、白细胞介素-8、白细胞介素-10、肿瘤坏死因子-α、生长调节致癌基因-α和调节激活正常T细胞表达和分泌因子，两组之间无显著差异（p≥0.05），而在鼻甲黏膜中发现白细胞介素-1β和白细胞介素-1受体拮抗剂的浓度显著更高（p≤0.05）。未检测到白细胞介素-3；仅偶尔发现粒细胞-巨噬细胞集落刺激因子。
本研究表明白细胞介素-5在嗜酸性鼻息肉的病理生理学中起关键作用，且可能由嗜酸性粒细胞产生。
