Kimura F, Baughman R W
Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
Eur J Neurosci. 1997 Jun;9(6):1137-43. doi: 10.1111/j.1460-9568.1997.tb01467.x.
In the mature cerebral cortex the interhemispheric connections across the corpus callosum appear to be essentially completely excitatory on the basis of both immunocytochemical and electrophysiological studies. During late embryonic development, however, immunocytochemical staining reveals numerous GABA-positive fibres in the callosum, which later largely disappear. The origin of these fibres and whether they represent functional GABAergic neurons has not been established. In the present study we used a combination of retrograde labelling in vivo with electrophysiology and immunocytochemistry in cell culture to show that transiently at birth in rat pups a substantial number of transcallosal cortical cells are functional GABAergic neurons. Possible roles and fates for these neurons are discussed.
基于免疫细胞化学和电生理学研究，在成熟的大脑皮层中，穿过胼胝体的半球间连接似乎基本上完全是兴奋性的。然而，在胚胎发育后期，免疫细胞化学染色显示胼胝体中有大量γ-氨基丁酸（GABA）阳性纤维，这些纤维后来大多消失。这些纤维的起源以及它们是否代表功能性GABA能神经元尚未确定。在本研究中，我们结合体内逆行标记、电生理学和细胞培养中的免疫细胞化学方法，以表明在新生大鼠幼崽出生时，大量胼胝体皮质细胞短暂地是功能性GABA能神经元。本文讨论了这些神经元可能的作用和命运。
