Merchenthaler I, Maderdrut J L, Cianchetta P, Shughrue P, Bronstein D
Laboratory of Molecular and Integrative Neuroscience, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA.
J Comp Neurol. 1997 Jul 28;384(2):211-32.
The distribution of preprodynorphin messenger RNA-containing perikarya in the central nervous system of the rat was determined with in situ hybridization histochemistry using a 35S-labelled complementary RNA probe. All of the regions of the central nervous system reported by other investigators to contain perikarya that synthesize prodynorphin-derived peptides, except the pedunculopontine tegmental nucleus, the accessory trigeminal nucleus, and the ventral nucleus of the trapezoid body, also contained perikarya that synthesize preprodynorphin messenger RNA. However, the olfactory bulb, the anterior olfactory nucleus, the islands of Calleja, the CA1-CA3 fields of the hippocampus, the septohippocampal nucleus, the diagonal band of Broca, the basal and cortical amygdaloid nuclei, the entopeduncular nucleus, the subthalamic nucleus, the superior colliculus, the Edinger-Westphal nucleus, the dentate nucleus, the raphes linearis and pontis, the dorsal cochlear nucleus, the medial vestibular nucleus, the inferior olive, and the dorsal motor nucleus of the vagus nerve also contained preprodynorphin messenger RNA-synthesizing perikarya. These observations suggest that prodynorphin-derived peptides have a much more pervasive role in central nervous system function than previously suspected. However, before the physiological significance of these observations can be judged, it will be necessary to determine whether all of the novel sites of preprodynorphin messenger RNA synthesis are sites of prohormone synthesis and conventional processing.
利用35S标记的互补RNA探针，通过原位杂交组织化学方法确定了大鼠中枢神经系统中含前强啡肽原信使核糖核酸的核周体的分布。其他研究者报道的中枢神经系统中所有含能合成强啡肽原衍生肽的核周体的区域，除了脚桥被盖核、三叉神经副核和斜方体腹侧核外，也都含有能合成前强啡肽原信使核糖核酸的核周体。然而，嗅球、前嗅核、卡勒雅岛、海马的CA1 - CA3区、隔海马核、布罗卡斜带、基底杏仁核和皮质杏仁核、内囊核、丘脑底核、上丘、动眼神经副核、齿状核、中缝线性核和脑桥核、背侧耳蜗核、内侧前庭核、下橄榄核以及迷走神经背运动核也都含有能合成前强啡肽原信使核糖核酸的核周体。这些观察结果表明强啡肽原衍生肽在中枢神经系统功能中的作用比以前认为的更为广泛。然而，在能够判断这些观察结果的生理学意义之前，有必要确定所有新的前强啡肽原信使核糖核酸合成位点是否都是激素原合成和常规加工的位点。
