Tsai S Y, McNulty J A
Department of Cell Biology, Neurobiology and Anatomy, Stritch School of Medicine, Loyola University of Chicago, Maywood, Illinois 60153, USA.
Glia. 1997 Jul;20(3):243-53. doi: 10.1002/(sici)1098-1136(199707)20:3<243::aid-glia8>3.0.co;2-8.
Microglia in the pineal gland of 1-day-old Sprague-Dawley rats were examined by OX-42 immunocytochemistry and DiI-acetylated-LDL uptake in pineal cell suspension and were found to comprise 3-5% of the total cells in the pineal gland of the neonates. In order to investigate the effects of microglia on pinealocyte structure and function, microglia-depleted and microglia-enriched pineal cell cultures were generated from 1-day-old neonate by fluorescence activated cell sorting (FACS). After 7 days of culture, tissues were processed for either immunocytochemistry for pinealocyte S-antigen and serotonin or high performance liquid chromatography to measure serotonin. Morphometric analysis of immunoreacted cells revealed that pinealocyte neurite length was enhanced in microglia-depleted cultures and was inhibited in a microglia-enriched environment (ANOVA, P < 0.001). Serotonin content of pineal cultures decreased in microglia-depleted cultures and was elevated in microglia-enriched cultures (ANOVA, P < 0.001) without any significant change in pinealocyte numbers. These findings are consistent with a working hypothesis that microglia function to mediate neuroendocrine-immune interactions of the gland.
采用OX - 42免疫细胞化学方法以及松果体细胞悬液中DiI - 乙酰化低密度脂蛋白摄取实验，对1日龄Sprague - Dawley大鼠松果体中的小胶质细胞进行了检测，结果发现小胶质细胞占新生大鼠松果体中细胞总数的3 - 5%。为了研究小胶质细胞对松果体细胞结构和功能的影响，通过荧光激活细胞分选技术（FACS）从1日龄新生大鼠中制备了小胶质细胞缺失和小胶质细胞富集的松果体细胞培养物。培养7天后，对组织进行处理，用于松果体细胞S抗原和5 - 羟色胺的免疫细胞化学检测，或用于高效液相色谱法测定5 - 羟色胺。对免疫反应细胞的形态计量分析显示，在小胶质细胞缺失的培养物中，松果体细胞的神经突长度增加，而在小胶质细胞富集的环境中则受到抑制（方差分析，P < 0.001）。在小胶质细胞缺失的培养物中，松果体培养物中的5 - 羟色胺含量降低，而在小胶质细胞富集的培养物中则升高（方差分析，P < 0.001），而松果体细胞数量没有任何显著变化。这些发现与一个工作假设一致，即小胶质细胞的功能是介导该腺体的神经内分泌 - 免疫相互作用。
