Lavoie J C, Chessex P
Research Center, Hôpital Ste-Justine; Department of Pediatrics, University of Montreal, Quebec, Canada.
Free Radic Biol Med. 1997;23(4):648-57. doi: 10.1016/s0891-5849(97)00011-7.
Gender and maturation affect glutathione status in human neonatal tissues. The objective was to verify if human tissues derived from baby girls had a greater ability then tissues derived from males to stimulate the glutathione-reductase, when faced with an oxidative challenge. In vitro, the effect of a calibrated oxidative challenge was studied in endothelial cells. In vivo, the effect of a clinically relevant oxidative challenge was studied in cells from tracheal aspirates derived from oxygen-dependent newborn infants. In endothelial cells, the oxidant tert-butylhydroperoxide had a stimulating effect on GSSG-R activity in cells derived from females. The peroxide produced a time, concentration and gender-dependent cytotoxicity, with female-derived cells exhibiting a better viability. In vivo, the intracellular total glutathione content was higher in female-derived cells and in cells from more mature babies; postnatal age and gestational age had a positive effect on the activity of GSSG-R. Oxygen (FiO2 > or = 0.3) was associated with a lower activity of GSSG-R in boys, early in life. Considering that glutathione is a central element in the antioxidant defense, these results suggest that specific tissues derived from the baby girl are potentially better protected against an oxidative stress than those derived from the boy.
性别和成熟度会影响人类新生儿组织中的谷胱甘肽状态。目的是验证当面临氧化应激时，来自女婴的人体组织是否比来自男婴的组织具有更强的刺激谷胱甘肽还原酶的能力。在体外，在校准的氧化应激条件下研究了内皮细胞的情况。在体内，在依赖氧气的新生儿气管吸出物的细胞中研究了临床相关氧化应激的影响。在内皮细胞中，氧化剂叔丁基过氧化氢对来自女性的细胞中的谷胱甘肽二硫化物还原酶（GSSG-R）活性有刺激作用。过氧化物产生了时间、浓度和性别依赖性的细胞毒性，来自女性的细胞表现出更好的活力。在体内，来自女性的细胞和更成熟婴儿的细胞中细胞内总谷胱甘肽含量更高；出生后年龄和胎龄对GSSG-R的活性有积极影响。在生命早期，氧气（吸入氧分数≥0.3）与男孩较低的GSSG-R活性相关。鉴于谷胱甘肽是抗氧化防御的核心要素，这些结果表明，来自女婴的特定组织可能比来自男婴的组织更能抵御氧化应激。
