通过测量mRNA总量进行快速杀细胞和细胞生长抑制化学敏感性试验。
Rapid cytocidal and cytostatic chemosensitivity test by measuring total amount of mRNA.
作者信息
Miura Y, deFries R, Shimada H, Mitsuhashi M
机构信息
Department of Pathology, University of California, Irvine, USA.
出版信息
Cancer Lett. 1997 Jun 24;116(2):139-44. doi: 10.1016/s0304-3835(97)00191-2.
Chemosensitivity of vinblastin, cisplatin, and mitomycin C were assessed in four different human cancer cell lines (U937, HL-60, CaR-1, and HepG2) by MTT (3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide) assay and the measurement of total cytosolic poly(A) + mRNA. Results of 12 h mRNA assay with 10 x peak plasma concentration (PPC) were significantly correlated with that of standard 3 days MTT assay with 1 x PPC. Furthermore, mRNA assay was changed more significantly than MTT assay under cytostatic condition. Because of its minimum culture requirement (12 h) and broad spectrum (cytocidal and cytostatic), mRNA assay will become a useful tool for chemosensitivity test.
通过MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）试验以及总细胞溶质聚腺苷酸加尾信使核糖核酸的测定，评估了长春碱、顺铂和丝裂霉素C对四种不同人类癌细胞系（U937、HL-60、CaR-1和HepG2）的化学敏感性。12小时信使核糖核酸试验（使用10倍峰值血浆浓度）的结果与标准的3天MTT试验（使用1倍峰值血浆浓度）的结果显著相关。此外，在细胞生长抑制条件下，信使核糖核酸试验的变化比MTT试验更显著。由于其最低培养要求（12小时）和广泛适用性（细胞杀伤和细胞生长抑制），信使核糖核酸试验将成为化学敏感性测试的一种有用工具。
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