Jager J, Tromp A, Hooymans J M, Reitsma W D, Smit A J
St. Lucas Ziekenhuis, Winschoten.
Ophthalmologica. 1997;211(4):209-13. doi: 10.1159/000310791.
We assessed the day-to-day reproducible of vitreous fluorophotometry in 7 type 1 diabetic patients and in 1 healthy control subject. The coefficient of variation for duplicate measurements was 33.7, 40.3 and 23.6%, for the right eyes, the left eyes and mean values of both eyes, respectively. Assessment of reproducibility in 1 healthy subject, measured 4 times, resulted in coefficients of variation of about 60%. These values are somewhat worse than those obtained by others in healthy subjects. Since technical problems do not seem to play a significant role in this regard, our reproducibility results reflect the true biological variability of the permeability of the blood-retina barrier assessed by vitreous fluorophotometry in diabetic patients. This variability is substantial. Therefore, we conclude that this method is not precise enough to be used in intervention studies with a limited number of subjects.
我们评估了7例1型糖尿病患者和1例健康对照者玻璃体荧光光度测定的每日可重复性。右眼、左眼及双眼平均值的重复测量变异系数分别为33.7%、40.3%和23.6%。对1例健康受试者进行4次测量的重复性评估，变异系数约为60%。这些值比其他研究在健康受试者中获得的值稍差。由于技术问题在这方面似乎不起重要作用，我们的重复性结果反映了糖尿病患者通过玻璃体荧光光度法评估的血视网膜屏障通透性的真实生物学变异性。这种变异性很大。因此，我们得出结论，该方法不够精确，不足以用于受试者数量有限的干预研究。
