Padilha-Gonçalves A
Universidade do Rio de Janeiro (UNI-RIO), Brazil.
Rev Inst Med Trop Sao Paulo. 1996 Jul-Aug;38(4):299-302. doi: 10.1590/s0036-46651996000400011.
The scales are collected by pressing small pieces of scotch tape (about 4 cm length and 2 cm width) onto the lesions and following withdrawal the furfuraceous scales will remain on the glue side. These pieces are then immersed for some minutes in lactophenol-cotton blue stain. Following absorption of the stain the scales are washed in current water to remove the excess of blue stain, dried with filter paper, dehydrated via passage in two bottles containing absolute alcohol and then placed in xylene in a centrifugation tube. The xylene dissolves the scotch tape glue and the scales fall free in the tube. After centrifugation and decantation the scales concentrated on the bottom of the tube are collected with a platinum-loop, placed in Canada balsam on a microscopy slide and closed with a cover slip. The preparations are then ready to be submitted to microscopic examination. Other stains may also be used instead of lactophenol-cotton blue. This method is simple, easily performed, and offers good conditions to study these fungi as well as being useful for the diagnosis of the diseases that they cause.
通过将小块透明胶带（约4厘米长、2厘米宽）按压在皮损上收集鳞屑，撕下后，糠状鳞屑会留在胶面上。然后将这些胶带片在石炭酸棉蓝染液中浸泡几分钟。吸去染液后，将鳞屑用流水冲洗以去除多余的蓝色染液，用滤纸吸干，依次通过两个装有无水乙醇的瓶子进行脱水，然后放入离心管中的二甲苯中。二甲苯溶解透明胶带的胶水，鳞屑在管中脱落。离心并倾析后，用白金环收集聚集在管底的鳞屑，置于载玻片上的加拿大树胶中，并用盖玻片覆盖。然后制备物即可进行显微镜检查。也可以使用其他染液代替石炭酸棉蓝。该方法简单、易于操作，为研究这些真菌提供了良好条件，也有助于诊断由它们引起的疾病。
