Mattern J, Koomägi R, Volm M
Department of Oncological Diagnostics and Therapy, German Cancer Research Center, Heidelberg, Germany.
Anticancer Res. 1997 May-Jun;17(3C):2249-52.
Tumor specimens from 84 patients with untreated epidermoid lung carcinomas were analysed immunohistochemically for the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), tumor cell proliferation (PCNA index) and tumor vascularity (vessel density). The purpose of this study was to find out whether differences in tumor cell proliferation and tumor vascularity might be associated with differential angiogenic growth factor expression. The present results indicate that the proliferation of the tumors is closely related to their expression of VEGF, but not for bFGF. The PCNA labelling index in VEGF positive tumors (VEGF+/bFGF- or VEGF+/bFGF+) was significantly higher than that in VEGF negative tumors (VEGF-/bFGF- or VEGF-/bFGF+; Wilcaxon rank sum test, p < 0.0001). To investigate whether VEGF or bFGF is involved in lung tumor angiogenesis, the data of VEGF and bFGF expression were correlated with vessel density. It was found that the expression of VEGF and bFGF were associated with increment of vessel density, however, vessel density was significantly increased only when VEGF and bFGF were coexpressed (p < 0.02). It is suggested that VEGF and bFGF might act cooperatively in the neovascularization of human epidermoid lung carcinomas.
对84例未经治疗的肺表皮样癌患者的肿瘤标本进行免疫组织化学分析，检测血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（bFGF）的表达、肿瘤细胞增殖（PCNA指数）和肿瘤血管生成（血管密度）。本研究的目的是确定肿瘤细胞增殖和肿瘤血管生成的差异是否可能与血管生成生长因子的差异表达有关。目前的结果表明，肿瘤的增殖与其VEGF的表达密切相关，而与bFGF无关。VEGF阳性肿瘤（VEGF+/bFGF-或VEGF+/bFGF+）中的PCNA标记指数显著高于VEGF阴性肿瘤（VEGF-/bFGF-或VEGF-/bFGF+；Wilcaxon秩和检验，p<0.0001）。为了研究VEGF或bFGF是否参与肺肿瘤血管生成，将VEGF和bFGF表达的数据与血管密度进行关联分析。发现VEGF和bFGF的表达与血管密度的增加有关，然而，只有当VEGF和bFGF共表达时血管密度才显著增加（p<0.02）。提示VEGF和bFGF可能在人肺表皮样癌的新生血管形成中协同作用。
