Hennig Y, Rogalla P, Wanschura S, Frey G, Deichert U, Bartnitzke S, Bullerdiek J
Center of Human Genetics and Genetic Counseling, University of Bremen, Germany.
Cancer Genet Cytogenet. 1997 Jul 15;96(2):129-33. doi: 10.1016/s0165-4608(96)00283-x.
Cytogenetic studies on uterine leiomyomas have shown that more than 60% of these tumors possess a normal karyotype and that 30% have clonal chromosomal aberrations. The most frequent changes are aberrations involving 12q14-15 and show rearrangements of the long arm of chromosome 7. Recently, we were able to demonstrate that in a variety of mesenchymal tumors showing 12q14-15 aberrations the HMGIC gene is rearranged thus playing a role in tumorigenesis. Here we report the results of HMGIC expression studies by RT-PCR of five uterine leiomyomas with different karyotypes. The RT-PCR studies were performed on two primary tumors showing a 12q14-15 aberration, one of which with an additional del(7) and three tumors with del(7) as the sole aberration. The tumor with the 12q14-15 aberration as the sole alteration and the leiomyoma with 12q14-15 rearrangement plus deletion of the long arm of chromosome 7 were shown to express HMGIC. In contrast, in all three tumors with the del(7) as the sole aberration no expression of HMGIC was noted.
对子宫平滑肌瘤的细胞遗传学研究表明，超过60%的此类肿瘤具有正常核型，30%有克隆性染色体畸变。最常见的变化是涉及12q14 - 15的畸变，并显示7号染色体长臂的重排。最近，我们能够证明，在各种显示12q14 - 15畸变的间叶性肿瘤中，HMGIC基因发生重排，从而在肿瘤发生中起作用。在此，我们报告通过RT - PCR对五个具有不同核型的子宫平滑肌瘤进行HMGIC表达研究的结果。RT - PCR研究在两个显示12q14 - 15畸变的原发性肿瘤上进行，其中一个伴有额外的del(7)，以及三个以del(7)作为唯一畸变的肿瘤。以12q14 - 15畸变作为唯一改变的肿瘤以及具有12q14 - 15重排加7号染色体长臂缺失的平滑肌瘤显示表达HMGIC。相反，在所有三个以del(7)作为唯一畸变的肿瘤中未观察到HMGIC的表达。
