Hill M A, Araya J C, Eckert M W, Gillespie A T, Hunt J D, Levine E A
Section of Surgical Oncology, Louisiana State University Medical Center and Stanley S. Scott Cancer Center, New Orleans 70112, USA.
Cancer. 1997 Jul 15;80(2):204-10. doi: 10.1002/(sici)1097-0142(19970715)80:2<204::aid-cncr6>3.0.co;2-t.
Recent studies have suggested that the Epstein-Barr virus (EBV) is associated with leiomyosarcoma in children with human immunodeficiency virus (HIV) and in organ transplant recipients. To determine whether EBV is associated with leiomyosarcoma in HIV negative patients, the authors examined resected leiomyosarcomas for EBV and HIV.
Twenty-four leiomyosarcomas were studied and their diagnosis confirmed on pathologic review. From these specimens DNA was isolated. Tumor samples were analyzed for EBV and HIV using a polymerase chain reaction (PCR) technique followed by gel electrophoresis and Southern blot analysis. DNA from an EBV-infected human Burkitt's lymphoma cell line and peripheral blood from an HIV positive patient were used as positive controls for the presence of EBV and HIV, respectively. Immunohistochemistry was performed using an antibody to Epstein-Barr nuclear antigen.
HIV was not present in any of the patients analyzed. EBV DNA was detected in tumor tissue; however, 80 cycles of PCR were used before EBV sequences were detected. Therefore, the data indicate that tumor tissue was not infected with EBV. The positive results observed after 80 cycles of PCR were likely due to infiltrating lymphocytes. Immunohistochemistry confirmed the lack of active or latent EBV infection in tumor cells.
The results indicate that EBV is not associated with sporadic leiomyosarcoma in HIV negative patients. Therefore, the biology of leiomyosarcoma associated with HIV may be substantially different from the more common sporadic form.
近期研究表明，爱泼斯坦-巴尔病毒（EBV）与人类免疫缺陷病毒（HIV）感染儿童及器官移植受者的平滑肌肉瘤有关。为确定EBV是否与HIV阴性患者的平滑肌肉瘤有关，作者对切除的平滑肌肉瘤进行了EBV和HIV检测。
研究了24例平滑肌肉瘤，并经病理检查确诊。从这些标本中提取DNA。采用聚合酶链反应（PCR）技术，随后进行凝胶电泳和Southern印迹分析，对肿瘤样本进行EBV和HIV检测。分别以EBV感染的人类伯基特淋巴瘤细胞系的DNA和HIV阳性患者的外周血作为EBV和HIV存在的阳性对照。使用抗爱泼斯坦-巴尔核抗原抗体进行免疫组织化学检测。
所有分析患者均未检测到HIV。在肿瘤组织中检测到EBV DNA；然而，在检测到EBV序列之前使用了80个PCR循环。因此，数据表明肿瘤组织未被EBV感染。80个PCR循环后观察到的阳性结果可能是由于浸润淋巴细胞所致。免疫组织化学证实肿瘤细胞中不存在活跃或潜伏的EBV感染。
结果表明，EBV与HIV阴性患者的散发性平滑肌肉瘤无关。因此，与HIV相关的平滑肌肉瘤的生物学特性可能与更常见的散发性形式有很大不同。
