Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, 7616914111, Kerman, Iran.
Research and Technology Institute of Plant Production (RTIPP), Shahid Bahonar University of Kerman, 7616914111, Kerman, Iran.
Virus Genes. 2021 Jun;57(3):293-301. doi: 10.1007/s11262-021-01835-y. Epub 2021 Apr 21.
Parsley severe stunt-associated virus (PSSaV) is a recently identified nanovirus first reported in Germany. During a survey for identification of nanoviruses infecting apiaceous plants in south-eastern Iran, PSSaV was identified and characterized using a combination of rolling circle amplification (RCA) and high-throughput sequencing. Parsley plant samples were collected from vegetable production farms in Kerman province. From two symptomatic samples (39Ba and 40Ba), seven PSSaV components (DNA-C, -S, -M, -R, -N, -U1 and -U2) with two phylogenetically distinct variants of DNA-R (R1 and R2) were identified. In common with the German isolate of PSSaV, no DNA-U4 component was identified. In addition, associated alphasatellite molecules were identified in samples 39Ba [n = 6] and 40Ba [n = 5]. Sequence analyses showed that concatenated component sequences of the two Iranian PSSaVs share 97.2% nucleotide identity with each other and 82% to the German isolate. The coat proteins (CPs) of the PSSaV Iranian sequences share 97.2% amino acid identity and ~ 84% identity with that of the German isolate. Sequence and phylogenetic analyses of a total of 11 recovered alphasatellites from the two samples can be classified into the genera Fabenesatellite [n = 2], Milvetsatellite [n = 1], Mivedwarsatellite [n = 2], Subclovsatellite [n = 2], Sophoyesatellite [n = 4] in the family Alphasatellitidae. Identification of PSSaV and other nanoviruses in wild and cultivated plants in Iran reveals that nanoviruses could be causing yield reduction in crops plants in this country.
欧芹严重矮缩相关病毒（PSSaV）是一种新发现的纳米病毒，最初在德国报道。在伊朗东南部对伞形科植物感染纳米病毒的调查中，使用滚环扩增（RCA）和高通量测序相结合的方法鉴定和表征了 PSSaV。从克尔曼省的蔬菜生产农场采集了欧芹植物样本。从两个有症状的样本（39Ba 和 40Ba）中，鉴定出了七个 PSSaV 成分（DNA-C、-S、-M、-R、-N、-U1 和 -U2），以及两种具有两个不同进化分支的 DNA-R（R1 和 R2）变体。与德国的 PSSaV 分离株一样，未鉴定出 DNA-U4 成分。此外，在样本 39Ba [n=6]和 40Ba [n=5]中还鉴定出了相关的阿尔法卫星分子。序列分析表明，两个伊朗 PSSaV 的串联成分序列彼此之间共享 97.2%的核苷酸同一性，与德国分离株的同源性为 82%。PSSaV 伊朗序列的衣壳蛋白（CP）彼此之间共享 97.2%的氨基酸同一性，与德国分离株的同源性约为 84%。对来自两个样本的总共 11 个回收阿尔法卫星的序列和系统发育分析可以分为 Fabenesatellite [n=2]、Milvetsatellite [n=1]、Mivedwarsatellite [n=2]、Subclovsatellite [n=2]、Sophoyesatellite [n=4]属在 Alphasatellitidae 科中。在伊朗的野生和栽培植物中鉴定出 PSSaV 和其他纳米病毒表明，纳米病毒可能导致该国作物产量下降。
