Quenelle D C, Keith K A, Dunleavy K E, Taylor B A, Bowdon B J, Brazier A D, Mullon C J, Allen L B
Life Sciences Division, Southern Research Institute, Lexington, MA 02173, USA.
Antiviral Res. 1997 Jul;35(2):123-9. doi: 10.1016/s0166-3542(97)00014-4.
We now report the confirmation of the work of Hollingshead et al. (1995) on development of a cell based hollow fiber (HF) system for evaluating potential anti-AIDS drugs in vivo using conventional mice rather than SCID mice. CD4 +, CEM-SS cells infected with HIV/1, strain RF, at a multiplicity of infection of 0.1 were placed into HFs. The fibers were implanted into the peritoneal cavity of outbred Swiss mice. Using this model, the antiviral activity of azidothymidine (AZT) at doses of approximately 150, 75 and 37.5 mg/kg/day was evaluated by administering AZT to the mice in drinking water. Upon fiber removal on day 6, AZT treatment was shown to significantly increase CEM cell viability over the untreated, virus control group and significantly reduced the levels of HIV p24 and HIV RT activity.
我们现在报告对霍林斯黑德等人（1995年）工作的验证，即开发一种基于细胞的中空纤维（HF）系统，用于在体内使用常规小鼠而非严重联合免疫缺陷（SCID）小鼠评估潜在的抗艾滋病药物。将感染HIV-1 RF株、感染复数为0.1的CD4 + CEM-SS细胞置于中空纤维中。这些纤维被植入远交群瑞士小鼠的腹腔。使用该模型，通过在饮用水中给小鼠施用叠氮胸苷（AZT），评估了剂量约为150、75和37.5 mg/kg/天的AZT的抗病毒活性。在第6天取出纤维时，结果显示，与未处理的病毒对照组相比，AZT治疗显著提高了CEM细胞活力，并显著降低了HIV p24水平和HIV逆转录酶（RT）活性。
