Hollingshead M, Roberson J, Decker W, Buckheit R, Elder C, Malspeis L, Mayo J, Grever M
Biological Testing Branch, DTP, DCT, NCI, Fairview Center, Frederick, MD 21701, USA.
Antiviral Res. 1995 Nov;28(3):265-79. doi: 10.1016/0166-3542(95)00055-q.
Previous studies demonstrated that human cell lines can be cultivated in hollow fibers in the subcutaneous and intraperitoneal compartments of mice. We have extended the range of cell lines to include cells infected with the human immunodeficiency virus (HIV). Furthermore, these HIV-infected cells have been shown to replicate in the hollow fibers located in both physiologic compartments (intraperitoneal and subcutaneous) of SCID mice. Treatment of the host mice with antiviral agents can suppress virus replication in these hollow fiber cultures. The potential use of this system for early in vivo screening of anti-HIV compounds is discussed.
先前的研究表明，人类细胞系可在小鼠皮下和腹腔内的中空纤维中培养。我们已将细胞系范围扩大到包括感染人类免疫缺陷病毒（HIV）的细胞。此外，这些感染HIV的细胞已被证明能在SCID小鼠的两个生理腔室（腹腔和皮下）中的中空纤维内复制。用抗病毒药物治疗宿主小鼠可抑制这些中空纤维培养物中的病毒复制。本文讨论了该系统在体内早期筛选抗HIV化合物方面的潜在用途。
