Gigli I, von Zabern I, Porter R R
Biochem J. 1977 Sep 1;165(3):439-46. doi: 10.1042/bj1650439.
The fourth component of complement, C4, was isolated from human serum in good yield, and in confirmation of previous reports was shown to be formed from three peptide chains, alpha, beta and gamma, with apparent mol.wts. 90 000, 80 000 and 30 000 respectively. Preparative methods are described for the isolation of the three peptide chains and their amino acid analyses reported. Component C4 contains 7.0% carbohydrate, alpha-chain 8.6% and the beta-chain 5.6%. The N-terminal amino acid sequences are given for 12 residues of the alpha-chain, eight of the beta-chain and 19 of the gamma-chain.
补体的第四成分C4已从人血清中大量分离出来，正如先前报道所证实的那样，它由三条肽链α、β和γ组成，其表观分子量分别为90000、80000和30000。本文描述了分离这三条肽链的制备方法，并报告了它们的氨基酸分析结果。成分C4含有7.0%的碳水化合物，α链含有8.6%，β链含有5.6%。给出了α链12个残基、β链8个残基和γ链19个残基的N端氨基酸序列。
