Andresen K, Gasim S, Elhassan A M, Khalil E A, Barker D C, Theander T G, Kharazmi A
Department of Clinical Microbiology, RHIMA University Hospital (Rigshospitalet), Copenhagen, Denmark.
Trop Med Int Health. 1997 May;2(5):440-4.
We have evaluated the sensitivity of the polymerase chain reaction (PCR) as a diagnostic tool for Leishmania donovani using blood, bone marrow and lymph node samples from Sudanese patients with a confirmed infection. Forty patients were diagnosed by microscopic examination of bone marrow or lymph node samples. The PCR was able to detect parasite DNA in 37 out of 40 blood samples. In bone marrow and lymph node samples, the PCR was able to detect parasite DNA in all 7 and 6 samples, respectively. We suggest that the PCR should be considered as a valuable and sensitive tool for the diagnosis of L. donovani infection. However, if PCR diagnosis is to supplement or even replace microscopic diagnosis in developing countries, a large number of patients with no apparent signs of infection and patients with other diseases have to be tested in order to evaluate its true potential.
我们使用来自苏丹确诊感染利什曼原虫的患者的血液、骨髓和淋巴结样本，评估了聚合酶链反应（PCR）作为杜氏利什曼原虫诊断工具的敏感性。通过对骨髓或淋巴结样本进行显微镜检查，确诊了40名患者。PCR能够在40份血液样本中的37份中检测到寄生虫DNA。在骨髓和淋巴结样本中，PCR分别能够在所有7份和6份样本中检测到寄生虫DNA。我们建议，PCR应被视为诊断杜氏利什曼原虫感染的一种有价值且敏感的工具。然而，如果要在发展中国家将PCR诊断作为显微镜诊断的补充甚至替代方法，就必须对大量无明显感染迹象的患者和患有其他疾病的患者进行检测，以评估其真正潜力。
