Yokozeki H, Katayama I, Ohki O, Arimura M, Takayama K, Matsunaga T, Satoh T, Umeda T, Azuma M, Okumura K, Nishioka K
Department of Dermatology, School of Medicine, Tokyo Medical and Dental University, Japan.
Br J Dermatol. 1997 Jun;136(6):831-7.
CD80 (B7-1) and CD86 (B7-2/B70) have recently been identified in cultured human Langerhans cells (LCs), although their role and regulatory properties remain unclear. We present our comparison of the expression of the molecules, mRNAs and the function between CD80 and CD86 in human LCs treated by interferon gamma (IFN-gamma). We examined the regulatory properties of CD80 and CD86 expression in human LCs pretreated with IFN-gamma. Flow cytometric analysis indicated that the mean fluorescence intensity of CD86 but not CD80 was enhanced. However, the percentage modulation of both CD80 and CD86 positive cells were significantly up-regulated in a dose-dependent manner, after 48-h culturing with IFN-gamma. The regulatory properties of CD80 and CD86 mRNA expressions in human LC were studied using polymerase chain reaction methods. We found that both CD80 and CD86 mRNA of enriched LCs following IFN-gamma pretreatment for 12 h were higher than those without pretreatment. We have demonstrated that the primary allogeneic mixed epidermal cell-lymphocyte reaction induced by human LCs treated by IFN-gamma increased in a dose-dependent manner. There was a 61.5% inhibition by anti-CD86 monoclonal antibody and a 32.5% inhibition by anti-CD80 monoclonal antibody. These data indicate that the CD80 and CD86 expression of human LCs may be differently regulated by IFN-gamma.
CD80（B7-1）和CD86（B7-2/B70）最近已在培养的人朗格汉斯细胞（LCs）中被鉴定出来，尽管它们的作用和调节特性仍不清楚。我们展示了在经γ干扰素（IFN-γ）处理的人LCs中，CD80和CD86在分子、mRNA表达及功能方面的比较。我们研究了用IFN-γ预处理的人LCs中CD80和CD86表达的调节特性。流式细胞术分析表明，CD86的平均荧光强度增强，而CD80未增强。然而，在用IFN-γ培养48小时后，CD80和CD86阳性细胞的百分比调节均以剂量依赖性方式显著上调。使用聚合酶链反应方法研究了人LC中CD80和CD86 mRNA表达的调节特性。我们发现，经IFN-γ预处理12小时后富集的LCs中，CD80和CD86 mRNA均高于未预处理的细胞。我们证明，经IFN-γ处理的人LCs诱导的原发性同种异体混合表皮细胞-淋巴细胞反应呈剂量依赖性增加。抗CD86单克隆抗体有61.5%的抑制作用，抗CD80单克隆抗体有32.5%的抑制作用。这些数据表明，人LCs的CD80和CD86表达可能受IFN-γ的不同调节。
