Renedo M, Arce I, Rodríguez A, Carretero M, Lanier L L, López-Botet M, Fernández-Ruiz E
Unidad de Biología Molecular, Hospital de la Princesa, Universidad Autónoma de Madrid, c/Diego de León 62, Madrid 28006, Spain.
Immunogenetics. 1997;46(4):307-11. doi: 10.1007/s002510050276.
Natural killer (NK) cells preferentially express several type II glycoproteins of the calcium-dependent lectin superfamily. The genes coding for these molecules are clustered on the distal mouse chromosome 6 and on the rat chromosome 4 in a region designated the NK gene complex. To date, no definite evidence of the presence of a NK gene complex has been found in humans. Here we report the assignment by fluorescence in situ hybridization of the CD94 gene to human chromosome 12p12-p13, in the same region where the CD69 and NKG2A genes had been previously mapped. In addition, using a yeast artificial chromosome contig spanning this region we determined that the human CD94, NKG2A, NKG2C, NKG2E, and NKR-P1A (NKR) genes map to the short arm of chromosome 12. The distal to proximal position of these loci are: NKR- CD69 - CD94/NKG2A/NKG2C/NKG2E. These data demonstrate the existence of a human NK gene complex located within a 5.6 cM interval flanked by the genetic markers D12S397 and D12S89. The physical distance spanned by the NK gene complex in humans ranges between 0.7 and 2.4 megabases.
自然杀伤（NK）细胞优先表达钙依赖性凝集素超家族的几种II型糖蛋白。编码这些分子的基因聚集在小鼠6号染色体远端以及大鼠4号染色体上一个被称为NK基因复合体的区域。迄今为止，尚未在人类中发现存在NK基因复合体的确切证据。在此，我们报告通过荧光原位杂交将CD94基因定位到人类12号染色体p12 - p13，该区域与先前已定位的CD69和NKG2A基因所在区域相同。此外，利用跨越该区域的酵母人工染色体重叠群，我们确定人类CD94、NKG2A、NKG2C、NKG2E和NKR - P1A（NKR）基因定位于12号染色体短臂。这些基因座从远端到近端的位置依次为：NKR - CD69 - CD94/NKG2A/NKG2C/NKG2E。这些数据证明在人类中存在一个位于遗传标记D12S397和D12S89两侧、间隔为5.6厘摩的NK基因复合体。人类NK基因复合体跨越的物理距离在0.7至2.4兆碱基之间。
