染色质核心颗粒的重建。

Reconstitution of chromatin core particles.

作者信息

Tatchell K, Van Holde K E

出版信息

Biochemistry. 1977 Nov 29;16(24):5295-303. doi: 10.1021/bi00643a021.

PMID:921932

Abstract

Chromatin core particles, containing 140 base pairs (bp) of DNA plus the inner histones, can be nearly quantitatively formed either by reassociation from 2 M NaCl or by reconstitution from salt extracted histones and DNA. The reassociated or reconstituted particles appear to be identical with the native particles in all physical properties examined (sedimentation velocity, histone content, circular dichroism, and melting) as well as in their patterns of digestion by micrococcal nuclease, DNase I, and trypsin. In the presence of excess DNA, no "half-particles" are formed. In the presence of excess histone, aggregated structures are formed in addition to 11S core particles.

摘要

染色质核心颗粒包含140个碱基对（bp）的DNA以及内部组蛋白，它几乎可以通过从2M氯化钠中重新缔合，或者从盐提取的组蛋白和DNA中重构而定量形成。重新缔合或重构的颗粒在所有检测的物理性质（沉降速度、组蛋白含量、圆二色性和熔解）以及它们被微球菌核酸酶、DNase I和胰蛋白酶消化的模式方面，似乎与天然颗粒相同。在过量DNA存在的情况下，不会形成“半颗粒”。在过量组蛋白存在的情况下，除了11S核心颗粒外，还会形成聚集结构。