Joshi A R, Sinha S, Sulaiman I M, Banerji A K, Hasnain S E
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.
Indian J Biochem Biophys. 1996 Dec;33(6):455-7.
We used a novel DNA fingerprinting probe O-chi-1 (ref. 1) to detect differences in the hybridization pattern of brain tumor DNA and paired normal tissue of a given individual. Representatives of meningiomas (two), glioblastoma multeforme (three) and astrocytoma (one) were studied. Alterations, which included amplification as well as the loss of a normal band in tumor DNA, were observed in four of the six tumours. While the increased intensity of a band can be taken to imply increased copy number, the disappearance of bands could either be due to loss of DNA sequence or rearrangement resulting in different sized bands.
我们使用一种新型DNA指纹图谱探针O-chi-1（参考文献1）来检测特定个体脑肿瘤DNA与配对正常组织杂交模式的差异。研究了脑膜瘤（2例）、多形性胶质母细胞瘤（3例）和星形细胞瘤（1例）的代表病例。在6个肿瘤中的4个中观察到改变，包括肿瘤DNA中正常条带的扩增以及缺失。虽然条带强度增加可被认为意味着拷贝数增加，但条带消失可能是由于DNA序列缺失或重排导致条带大小不同。
