Kremer J M, Wiersema P H
Biochim Biophys Acta. 1977 Dec 15;471(3):348-60. doi: 10.1016/0005-2736(77)90041-4.
Processes occurring in dispersions of dimyristoyl phosphatidylcholine containing myristic acid have been studied by light scattering of dilute dispersions (concn. less than or equal to 1 mg/ml) at temperatures above and below the phase transition temperatures of these dispersions. The transition temperatures increase with increasing mol fraction of myristic acid. Above these temperatures, vesicles with different mol fractions of myristic acid exchange lipid molecules. The exchange process leads to vesicles having phase transition temperatures and radii, which ar both intermediate between the initial transitions and radii, respectively. In contrast with the observations above the phase transitions, it was found that when dimyristoyl phosphatidylcholine/myristic acid vesicles were cooled to a few degrees below the phase transition, larger particles were formed. These observations are consistent with a mechanism consisting of vesicle aggregation followed by fustion of the aggregated vesicles. The aggregation process is of second order in the vesicle concentration, and its rate increases with increasing mol fraction of myristic acid.
通过在这些分散体系的相变温度之上和之下的温度下，对稀分散体系（浓度小于或等于1mg/ml）进行光散射，研究了含有肉豆蔻酸的二肉豆蔻酰磷脂酰胆碱分散体系中发生的过程。相变温度随着肉豆蔻酸摩尔分数的增加而升高。在这些温度之上，具有不同肉豆蔻酸摩尔分数的囊泡会交换脂质分子。交换过程导致囊泡具有相变温度和半径，其分别介于初始相变温度和半径之间。与相变温度之上的观察结果相反，发现当二肉豆蔻酰磷脂酰胆碱/肉豆蔻酸囊泡冷却至相变温度以下几度时，会形成更大的颗粒。这些观察结果与由囊泡聚集随后聚集的囊泡融合组成的机制一致。聚集过程在囊泡浓度上是二级反应，其速率随着肉豆蔻酸摩尔分数的增加而增加。
