一种来自人宫颈癌细胞系（HeLa细胞）的胞质外切核糖核酸酶。

A cytoplasmic exoribonuclease from HeLa cells.

作者信息

Kwan C N

出版信息

Biochim Biophys Acta. 1977 Dec 2;479(3):322-31. doi: 10.1016/0005-2787(77)90114-9.

PMID:922003

Abstract

An exoribonuclease has been purified from the cytoplasm of HeLa cells. The enzyme produces 5'-AMP as the only product from poly(A). The degradation proceeds in a 3' to 5' direction, and a 3'-OH terminus is required. In addition to poly(A), the enzyme degrades other synthetic homopolymers as well as natural messenger, and ribosomal RNAs. The enzyme can also degrade the poly(A) tract of messenger RNA. DNA and double-stranded RNA are resistant to the enzyme.

摘要

一种外切核糖核酸酶已从HeLa细胞的细胞质中纯化出来。该酶以聚腺苷酸（poly(A)）为底物时，仅产生5'-AMP作为产物。降解反应沿3'至5'方向进行，且需要3'-OH末端。除了聚腺苷酸，该酶还能降解其他合成均聚物以及天然信使RNA和核糖体RNA。该酶也能降解信使RNA的聚腺苷酸序列。DNA和双链RNA对该酶具有抗性。