Kumar R, Taneja V A
Biochim Biophys Acta. 1977 Dec 8;485(2):489-91. doi: 10.1016/0005-2744(77)90184-x.
Until recently there were no reports regarding the presence of xanthine oxidase (xanthine:oxygen oxidoreductase, EC 1.2.3.2) in plants. Direct evidence for its presence in lentil seedlings is reported here. Xanthine oxidase activity increases with the period of germination, reaching a maximum at 24 h and decreasing thereafter. The pH optimum for its activity is at pH 8.0. Almost equal activity is observed against xanthine and hypoxanthine. The Km for xanthine is 1.05 mM, and considerable inhibition is observed at high substrate concentration.
直到最近，还没有关于植物中存在黄嘌呤氧化酶（黄嘌呤：氧氧化还原酶，EC 1.2.3.2）的报道。本文报道了其在小扁豆幼苗中存在的直接证据。黄嘌呤氧化酶活性随萌发时间增加，在24小时达到最大值，之后下降。其活性的最适pH为8.0。对黄嘌呤和次黄嘌呤的活性几乎相同。黄嘌呤的Km为1.05 mM，在高底物浓度下观察到显著抑制。
