牛奶黄嘌呤氧化酶催化次黄嘌呤氧化的动力学研究。
A kinetic study of hypoxanthine oxidation by milk xanthine oxidase.
作者信息
Escribano J, Garcia-Canovas F, Garcia-Carmona F
机构信息
Departamento de Bioquimica, Universidad de Murcia, Spain.
出版信息
Biochem J. 1988 Sep 15;254(3):829-33. doi: 10.1042/bj2540829.
Abstract
The course of the reaction sequence hypoxanthine----xanthine----uric acid catalysed by xanthine:oxygen oxidoreductase from milk was investigated on the basis of u.v. spectra taken during the course of hypoxanthine and xanthine oxidations. It was found that xanthine accumulated in the reaction mixture when hypoxanthine was used as a substrate. The time course of the concentrations of hypoxanthine, xanthine intermediate and uric acid product was simulated numerically. The mathematical model takes into account the competition of substrate, intermediate and product and the accumulation of the intermediate at the enzyme. This type of analysis permits the kinetic parameters of the enzyme for hypoxanthine and xanthine to be obtained.
摘要
基于在次黄嘌呤和黄嘌呤氧化过程中获取的紫外光谱，对由牛奶中的黄嘌呤：氧氧化还原酶催化的反应序列次黄嘌呤→黄嘌呤→尿酸进行了研究。发现当使用次黄嘌呤作为底物时，黄嘌呤在反应混合物中积累。对次黄嘌呤、黄嘌呤中间体和尿酸产物浓度的时间进程进行了数值模拟。该数学模型考虑了底物、中间体和产物之间的竞争以及中间体在酶处的积累。这种分析类型能够获得该酶对次黄嘌呤和黄嘌呤的动力学参数。
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