Weaver C
Department of Public Health, Medical College, Fu Jen Catholic University, Hsingchuang, Taipei Hsien, Taiwan, Republic of China.
Microsc Res Tech. 1997;37(5-6):520-42. doi: 10.1002/(SICI)1097-0029(19970601)37:5/6<520::AID-JEMT14>3.0.CO;2-S.
The pancreas has a complex vasculature which comprises both exocrine and endocrine structures. Copper deficiency induces highly selective acinar cell degeneration and progressive noninflammatory lipomatosis in pancreas while Langerhans islets, ducts, and nerves remain unaffected. Pancreatic vasculature was examined in rats that had dietary copper deficiency to characterize changes in the angioarchitecture of the gland. This model was used to assess the degree to which the vasculature of non-acinar components of the gland are potentially altered under conditions of exocrine atrophy. Ultrastructure of pancreas was examined by histology, enzyme histochemistry and immunohistochemistry, corrosion casting and scanning electron microscopy, in situ vascular staining, microsphere injection, biochemical analysis, and morphometry in copper-deficient rats. Results show that no acute angiopathic changes indicative of vascular disorganization accompany atrophy. Only a reduction in the complexity of the capillary beds, which normally vascularize the dense acinar parenchyma, was found. Microsphere quantitation also showed that blood flow to the lipomatous gland remains intact. Furthermore, analysis of the angioarchitecture of the atrophied pancreas supports a largely autonomous blood supply to islets and ducts. These observations support the hypothesis that while the vasculature of the atrophied gland is modified in vascular regions severely targeted by acinar necrosis, the overall structural features of the angioarchitecture are preserved. The atrophied gland thus provides an experimental model to study the vascular routes supplying islet and ductal blood flow within the complex pancreatic circulation.
胰腺具有复杂的脉管系统，该系统包含外分泌和内分泌结构。铜缺乏会导致胰腺中高度选择性的腺泡细胞变性和进行性非炎性脂肪瘤形成，而胰岛、导管和神经则不受影响。对饮食中缺乏铜的大鼠的胰腺脉管系统进行了检查，以表征该腺体血管结构的变化。该模型用于评估在胰腺外分泌萎缩的情况下，腺体非腺泡成分的脉管系统可能发生改变的程度。通过组织学、酶组织化学和免疫组织化学、铸型腐蚀和扫描电子显微镜、原位血管染色、微球注射、生化分析以及对缺铜大鼠的形态计量学检查胰腺的超微结构。结果表明，萎缩过程中没有伴随血管紊乱的急性血管病变。仅发现正常为密集腺泡实质提供血液供应的毛细血管床的复杂性降低。微球定量分析还表明，流向脂肪瘤样腺体的血流保持完整。此外，对萎缩胰腺血管结构的分析支持胰岛和导管在很大程度上有自主的血液供应。这些观察结果支持这样的假设，即虽然萎缩腺体的脉管系统在受到腺泡坏死严重影响的血管区域发生了改变，但血管结构的整体特征得以保留。因此，萎缩的腺体提供了一个实验模型，用于研究复杂胰腺循环中为胰岛和导管提供血流的血管途径。
